摘要
目的:研究山茱萸不同部位(果肉、果核和全果)提取物对醋氨酚所致小鼠急性肝损伤的保护作用,为其全面开发利用提供参考。方法:将120只小鼠随机分为正常对照组(生理盐水)、模型组(生理盐水)、联苯双酯组(阳性对照,150 mg/kg)和山茱萸果肉、果核、全果提取物的低、中、高剂量组(以生药量计为1.56、6.24、12.48 g/kg),每组10只。灌胃给药,每天1次,连续7 d。末次给药后6 h,正常对照组小鼠腹腔注射生理盐水,其余各组小鼠腹腔注射醋氨酚300 mg/kg制备肝损伤模型。成模16 h后,测定小鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、丙二醛(MDA)以及肝组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)水平,苏木精-伊红染色后观察小鼠肝组织病理变化。结果:与正常对照组比较,模型组小鼠血清中ALT、AST、MDA水平显著升高(P<0.01),肝组织中SOD、GSH-Px水平显著降低(P<0.01);肝组织可见炎性细胞浸润,伴随肝细胞变性、片状坏死。与模型组比较,山茱萸果肉提取物中剂量组小鼠血清中ALT、AST、MDA水平和山茱萸果肉提取物高剂量组小鼠血清中ALT、MDA水平显著降低(P<0.05或P<0.01),山茱萸果核提取物低剂量组小鼠血清中MDA水平和山茱萸果核提取物中、高剂量组小鼠血清中ALT、AST水平均显著降低(P<0.05或P<0.01),山茱萸全果提取物中、高剂量组小鼠血清中ALT、AST水平均显著降低(P<0.05或P<0.01);山茱萸不同部位提取物中、高剂量组小鼠肝细胞坏死均有不同程度减轻,其中山茱萸果核提取物中、高剂量组和山茱萸全果高剂量组小鼠肝小叶结构基本完整,未出现肝细胞片状坏死现象。结论:山茱萸不同部位提取物对醋氨酚所致急性肝损伤模型小鼠均具有保护作用,且以果核提取物作用最好;其保肝机制可能与抗氧化应激反应有关。
OBJECTIVE:To study the protective effects of different parts(fruit pulp,fruit core,whole fruit)extracts of Cornus officinalis on acetaminophen-induced acute liver injury in mice,and to provide reference for its complete development and utilization.METHODS:Totally 120 mice were randomly divided into normal control group(normal saline),model group(normal saline),bifendate group(positive control,150 mg/kg),low-dose,medium-dose and high-dose groups(1.56,6.24,12.48 g/kg,calculated by crude drug)of C.officinalis fruit pulp,fruit core,whole fruit extracts,with 10 mice in each group.They were given relevant medicine intragastrically once a day for consecutive 7 d.6 h after last medication,normal control group was given normal saline intraperitoneally,and other groups were given acetaminophen 300 mg/kg intraperitoneally to induce liver injury model.16 h after modeling,the levels of ALT,AST and MDA in serum as well as the levels of GSH-Px and SOD in liver tissue were determined.HE staining was used to observe the pathological changes of liver tissue in mice.RESULTS:Compared with normal control group,the serum levels of ALT,AST and MDA in model group were increased significantly(P<0.01),while the levels of SOD and GSH-Px in liver tissue were decreased significantly(P<0.01);the inflammatory cell infiltration was observed in the liver tissue of the model group,accompanied by hepatocyte degeneration and flaky necrosis.Compared with model group,the serum levels of ALT,AST and MDA in C.officinalis fruit pulp extracts medium-dose group as well as the serum levels of ALT and MDA in C.officinalis fruit pulp extracts high-dose group were decreased significantly(P<0.05 or P<0.01).Serum level of MDA in C.officinalis fruit core extracts low-dose group,the serum levels of ALT and AST in C.officinalis fruit core extracts medium-dose and high-dose groups were decreased significantly(P<0.05 or P<0.01).The serum levels of ALT and AST were decreased significantly in C.officinalis fruit extracts medium-dose and high-dose groups(P<0.05 or P<0.01).The necrosis of liver cells was relieved in different parts of extracts C.officinalis middle-dose and high-dose groups to different extent.The structure high-dose groups,C.officinalis fruit extracts high-dose group.CONCLUSIONS:Different parts extracts of C.officinalis have protective effects against acetaminophen-induced acute liver injury model mice,and the effect of C.officinalis fruit core extracts is similar to that of C.officinalis fruit extracts.The mechanism of hepatoprotection may be related to anti-oxidative stress reaction.
作者
南美娟
唐凯
张化为
崔春利
邓翀
NAN Meijuan;TANG Kai;ZHANG Huawei;CUI Chunli;DENG Chong(College of Pharmacy,Shaanxi University of TCM,Shaanxi Xianyang 712046,China)
出处
《中国药房》
CAS
北大核心
2018年第17期2385-2389,共5页
China Pharmacy
基金
陕西省社会发展科技攻关项目(No.2015SF246)
关键词
山茱萸
不同部位
醋氨酚
急性肝损伤
保肝作用
氧化应激
小鼠
Cornus officinalis
Different parts
Acetaminophen
Acute liver injury
Liver protection
Oxidative stress
Mice
