摘要
目的探讨复方阿胶浆对小鼠子宫内膜的作用。方法将雌鼠随机分为4组,分别为假手术组、假手术中药组、去势组及去势中药组,假手术中药组及去势中药组予复方阿胶浆灌胃处理。分别采用HE染色、免疫组化、免疫印迹实验法来分析复方阿胶浆对小鼠子宫内膜的作用。结果与假手术组相比,假手术中药组小鼠子宫增大,内膜增厚,腺体数量增多,子宫内膜血管内皮生长因子A(vascular endothelial growth factor-A,VEGF-A)表达量增多(P<0.05);去势中药组与去势组相比差异无显著性。结论复方阿胶浆可促进假手术组小鼠子宫内膜的生长,并可改善子宫内膜容受性,其作用机制可能是通过调节卵巢功能而间接作用于子宫。
Objective To explore the effect of compound Ejiao slurry on mouse uterus.Methods Female mice were randomly divided into four groups:sham operation group,sham operation with Chinese medicine group,ovariectomized group,and ovariectomized with Chinese medicine group.The sham operation with Chinese medicine group and ovariectomized with Chinese medicine group were treated with compound Ejiao slurry gavage.HE staining,immunohistochemical staining,and Western blot were used to observe and compare the endometrium among the groups.Results Compared with that of the sham operation group,the endometrium of the sham operation with Chinese medicine group was thicker.In addition,the number of glands and the expression of vascular endothelial growth factor-A(VEGF-A)in endometrium slightly increased(P<0.05).However,the differences between the ovariectomized group and ovariectomized with Chinese medicine group were not significant.Conclusions The compound Ejiao slurry can affect the growth of reproductive organs in female mice.It may regulate the endometrium by acting on the ovary.
作者
丁桂清
魏华芳
王立群
朱楚超
王晶
DING Guiqing;WEI Huafang;WANG Liqun;ZHU Chuchao;WANG Jing(Hubei University of Chinese Medicine,Wuhan 430070,China;Department of Gynaecology and Obstetrics,Wuhan General Hospital of Chinese People’s Liberation Army,Wuhan 430070)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第9期27-32,共6页
Chinese Journal of Comparative Medicine
基金
湖北省卫计委基金项目(编号:WJ2017M162)
关键词
复方阿胶浆
子宫内膜
血管内皮生长因子A
compound Ejiao slurry
endometrium
vascular endothelial growth factor-A(VEGF-A)
