摘要
[目的]检测贵阳市河水中大肠杆菌O157:H7。[方法]通过大肠杆菌O157:H7毒力基因志贺样毒素2(stx2)、α-溶血素(hly A)、紧密黏附素(eae A)设计3对引物,并建立了实时荧光定量PCR检测方法对8株菌株进行特异性、灵敏度检测,利用该方法检测了采集自贵阳市部分河流10组水样。[结果]该方法能够特异扩增大肠杆菌O157:H7毒力基因,灵敏度为127 ng/m L;检测水样结果与国标方法相比,其灵敏度为87.5%,特异度为97.1%,准确度为94.0%。[结论]该方法的建立能够为大肠杆菌O157:H7检测提供较好的理论基础。
[Objective]The research aimed to detect the Escherichia coli O157:H7 from river water of Guiyang City[Method]3 pairs of primers were designed by using E.coli O157:H7 virulence gene Shiga toxin 2(Stx2),alpha hemolysin(hlyA)and tight adhesin(eaeA).Quantitative real-time PCR assay was established to detect the specificity and sensitivity of the 8 strains.10 water samples collected from some rivers in Guiyang City were detected by this method.[Result]The method could amplify the virulence gene of E.coli O157:H7 and the sensitivity was 127 ng/mL.Compared with the national standard method,the sensitivity of the detected water sample was 87.5%,the specificity was 97.1%,and the accuracy was 94.0%.[Conclusion]The establishment of the method can provide a good theoretical basis for the detection of E.coli O157:H7.
作者
袁光宇
龚维瑶
贾玲玲
程茹
谢体波
钟新敏
YUAN Guang-yu;GONG Wei-yao;JIA Ling-ling(Guizhou Kwinbon Science and Technology for Food Safety Co.,Ltd.,Guiyang,Guizhou 550009)
出处
《安徽农业科学》
CAS
2018年第28期167-168,185,共3页
Journal of Anhui Agricultural Sciences
基金
贵州省科技计划项目(黔科合[2016]支撑2908号)
贵州省工业和信息化发展专项(2017002)
贵阳市人才创新创业项目(筑人才办合同字[2016]第13号)
贵阳市科技计划项目(黔科合成果[2018]4323)
