摘要
通过制备纳米金颗粒-适体结构,结合智能手机数字比色法实现了磺胺类抗生素磺胺二甲嘧啶(SDM)的快速定量检测。核酸适体通过静电作用结合在纳米金颗粒表面,可通过改变纳米金颗粒表面的电荷密度,使纳米金无法聚集,结合适体链的纳米金溶液呈现稳定的红色;目标分子与适体链特异性结合后,导致适体链从纳米金颗粒表面脱离,进而纳米金颗粒发生聚集,溶液颜色由红色变成蓝紫色。利用智能手机获取检测溶液的数字化图片,并用自行设计开发的手机App对图片的补色波长进行分析,可实现对SDM的定量检测。该方法检测范围为0~5μg/m L,检出限为0.13μg/m L。
A smartphone digital colorimetric method was proposed for the rapid detection of sulfonamides based on the aptamer/AuNPs complexation structure.Aptamers were bound on the surfaces of AuNPs by the electrostatic interaction which changed the charge density of the nanoparticles surfaces and the complexation of AuNPs with aptamers,preventing the nanoparticles from aggregating in a high-salt solution.The solution kept a stable red.In the presence of sulfonamides,the weaker binding between aptamers and the AuNPs was broken,and sulfonamides bound up with aptamers.The specific and stronger binding between sulfonamides and the aptamers led to the separation of the aptamers from the surfaces of the nanoparticles.The nanoparticles began to aggregate,which could result in a color changed from red to purple-blue.In order to achieve a precise,quantitative,fast and convenient detection,a smartphone was arranged to capture the digital images.The complementary wavelength of images was analyzed by a smartphone App,and the concentration of sulfadimethoxine(SDM)in the range of 0-5μg/mL could be determined by analyzing the correlation between SDM concentration and the complementary wavelength.The method showed high sensitivity,and the detection limit was 0.13μg/mL.
作者
王乐
李海琴
王美玲
张校亮
李晓春
WANG Le;LI Hai-qin;WANG Mei-ling;ZHANG Xiao-liang;LI Xiao-chun(College of Physics and Optoelectronics,Key Laboratory of Advanced Transducers and Intelligent Control System, Ministry of Education and Shanxi Province,Taiyuan University of Technology,Taiyuan 030024,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第8期967-971,共5页
Journal of Instrumental Analysis
基金
国家自然科学基金项目(21575098，21505098，81740195，61775157)；山西省平台基地和人才专项-优秀人才科技创新项目(201605D211033)；山西省应用基础研究面上青年基金项目(201701D221072)
关键词
纳米金团聚
数字比色法
适体
抗生素检测
智能手机App
nanoparticles aggregation
digital colorimetric method
aptamers
antibiotics detection
smartphone App
