摘要
建立了QuEChERS-气相色谱-质谱联用(GC-MS)法同时测定人参中20种农药残留量的检测技术。样品前处理采用改进的QuEChERS方法,人参粉加水浸润后,用乙腈均质提取,用N-丙基乙二胺(PSA)和MgSO_4净化,结合GC-MS检测系统,在电子轰击源(EI)选择离子监测(SIM)模式下进行检测,使用HP-5毛细管柱进行分离。在优化条件下, 20种农药在一定范围内线性关系良好,相关系数大于0. 990,方法检出限(信噪比S/N=3)为0. 001～0. 007 mg/kg,定量限(S/N=10)为0. 002～0. 024 mg/kg,平均回收率为70. 41%～114. 06%,相对标准偏差(RSD)为0. 76%～15. 47%。该方法操作简单快捷、灵敏度高。
In this study,the extraction and purification improvements of the QuEChERS method were coupled with gas chromatography-mass spectrometry(GC-MS)to determine 20 pesticides in panax ginseng.Dry ginseng powder was mixed with water,and the solution was then extracted with acetonitrile and purified with N-propylethylenediamine(PSA)and MgSO 4 to remove co-extractives that might interfere with the results.The target compounds were detected after electron-impact ionization in selected ion monitoring(SIM)mode.Under optimum conditions,the method gave a good linear relationship for the determination of the pesticides within a certain concentration range,with correlation coefficients greater than 0.990.Moreover,the limits of detection(S/N=3)were 0.002-0.007 mg/kg,the limits of quantification(S/N=10)were 0.002-0.024 mg/kg,and the average recoveries for pesticides in panax ginseng were 70.41%-114.06%,with relative standard deviations of 0.76%-15.47%.In comparison with previous methods,the new procedure has the advantages of simple sample preparation and higher sensitivity.
作者
崔丽丽
闫梅霞
朴向民
逄世峰
王英平
CUI Lili;YAN Meixia;PIAO Xiangmin;PANG Shifeng;WANG Yingping(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences, Changchun 130112,China)
出处
《色谱》
CAS
CSCD
北大核心
2018年第11期1173-1180,共8页
Chinese Journal of Chromatography
基金
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)
吉林省科技发展计划项目(20170309007YY
20170623038TC)~~
关键词
QUECHERS
气相色谱-质谱
农药
人参
QuEChERS
gas chromatography-mass spectrometry(GC-MS)
pesticides
panax ginseng
