摘要
目的:研究盐酸纳洛酮对急性呼吸窘迫综合征(ARDS)大鼠的免疫保护作用及其机制。方法:将30只健康SD大鼠随机分为对照组、模型组和纳洛酮组,每组10只。对照组:股静脉注射等量生理盐水;模型组:股静脉注射油酸(0. 15 ml·kg^(-1)),2 h后注射生理盐水(1. 0 ml·kg^(-1));纳洛酮组:股静脉注射油酸(0. 15 ml·kg^(-1)),2 h后股静脉注射1. 0 ml·kg^(-1)盐酸纳洛酮。计算左肺湿干比重,测定血浆中β-内啡肽、T淋巴细胞亚群含量,采用酶联免疫吸附法(ELISA)检验右肺匀浆TNF-α、IL^(-1)β、IL-6、IL-8含量。结果:与对照组比较,ARDS模型组大鼠的左肺湿干比重、β-内啡肽、CD3+T细胞、CD4+T细胞、CD4+/CD8+T细胞、TNF-α、IL^(-1)β、IL-6、IL-8水平均显著升高,CD8+T细胞显著降低(P <0. 05)。与ARDS模型组比较,纳洛酮组大鼠的左肺湿干比重、β-内啡肽、CD3+T细胞、CD4+T细胞、CD4+/CD8+T细胞、TNF-α、IL^(-1)β、IL-6、IL-8水平均显著降低,CD8+T细胞显著升高(P <0. 05)。结论:盐酸纳洛酮通过抑制血浆β-内啡肽升高调节T淋巴细胞亚群平衡,降低机体炎症反应,减轻肺泡水肿,对ARDS起保护作用。
Objective:To investigate the immune protection and mechanism of naloxone hydrochloride on rats with acute respiratory distress syndrome(ARDS).Methods:Thirty healthy SD rats were randomly divided into control group,model group,and naloxone group,each group had ten rats.Control group:1.15 ml·kg-1 normal saline was administrated by femoral vein injection.Model group:1.0 ml·kg-1 normal saline was administrated by femoral vein injection after 0.15 ml·kg-1 oleic acid was administrated.Naloxone group:1.0 ml·kg-1 naloxone hydrochloride was administrated by femoral vein injection after 0.15 ml·kg-1 oleic acid was administrated.The ratio of left lung wet/dry weight was calculated.β-endorphins and T-lymphocyte subsets in plasma was determined,and the content of TNF-α,IL-1β,IL-6,IL-8 were determined by enzyme linked immunosorbent assay(ELISA).Results:Compared with control group,Left lung wet/dry weight ratio,the level ofβ-endorphins,CD3+,CD4+,CD4+/CD8+,TNF-α,IL-1β,IL-6,IL-8 of rats in ARDS model group were significantly increased,CD8+was significantly lower than control group(P<0.05).Compared with model group,Left lung wet/dry weight ratio,the level ofβ-endorphins,CD8+,TNF-α,IL-1β,IL-6,IL-8 of rats in naloxone group were significantly decreased,CD3+,CD4+and CD4+/CD8+were significantly increased(P<0.05).Conclusion:Naloxone hydrochloride can protect body from ARDS by inhibiting plasmaβ-Endorphins,adjecting T-lymphocyte subsets,reducing body inflammation and alveolar edema.
作者
冯敏
刘刚
徐大千
FENG Min;LIU Gang;XU Daqian(Department of Critical Care Medicine,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《东南大学学报（医学版）》
CAS
2018年第5期824-828,共5页
Journal of Southeast University(Medical Science Edition)
基金
河南省教育厅项目(15A320065)
