摘要
去甲斑蝥素(NCTD)是斑蝥素(CTD)的衍生物,可人工合成。去甲斑蝥素对害虫有毒杀作用。为明确它是否有拒食作用及其作用方式,本研究以小菜蛾幼虫为供试昆虫,采用叶碟浸液法对试虫拒食作用及取食量、取食选择、取食次数和持续时间等取食行为进行了生物测定。拒食作用结果显示,2-48 h内NCTD处理组的幼虫取食量明显低于对照组且两者差距随时间逐渐增加,拒食率与剂量呈显著正相关,增加剂量会增加拒食率。行为研究结果显示,小菜蛾处理组试虫的单次平均取食时长明显减少,仅为对照组的70. 6%,而持续取食次数和试探次数均明显增多,分别是对照组的1. 4倍和1. 9倍。结果表明,NCTD对小菜蛾幼虫的拒食作用主要表现在降低试虫的取食量,减少取食时间,增加试探次数。
Norcantharidin(NCTD)is a derivative of cantharidin(CTD),which can be artificially synthesized and has a toxic effect on pests.In order to clarify its antifeedant effect and mode of action,this study took the arvae of diamondback moth(Plutella xylostella(L.))as test insect,leaf dish dipping method was used to test the antifeeding effect,food intake,feeding selection and feeding times.Antifeeding effect results displayed that the food intake of NCTD treatment group was significantly lower than that of the control group at 2-48 h,and the gap increased with time.There was a significant positive correlation between antifeedant rate and dose,and increasing the dose increased antifeedant rate.The results of feeding behavior showed that,the single average feeding time of the NCTD treatment group was significantly reduced,only 70.6%of the control group,while the number of continuous feed and number of trial was increased significantly,1.4 times and 1.9 times of the control group,respectively.The results showed that the antifeedant effect of NCTD on the P.xylostella larvae was mainly reflected in the reduction of the feeding amount,the reduction of feeding time,and the increase of trials number.
作者
李元盛
董兆克
张志勇
王进忠
LI Yuan-Sheng;DONG Zhao-Ke;ZHANG Zhi-Yong;WANG Jin-Zhong(Beijing Key Laboratory of New Technology in Agricultural Application,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)
出处
《环境昆虫学报》
CSCD
北大核心
2018年第5期1191-1196,共6页
Journal of Environmental Entomology
基金
国家重点研发计划(2017YFD0200307)
北京市粮经作物产业创新团队(BAIC09-2017)
关键词
去甲斑蝥素
小菜蛾
拒食中浓度
取食行为
Norcantharidin
Plutella xylostella
antifeedant concentration(AFC 50)
feeding behavior
