摘要
目的探讨风药(防风、白芷)对肝阳上亢型肾性高血压大鼠的降压增效作用及其机理。方法将100只雄性SD大鼠,随机分为正常组(12只)、手术组(76只)、假手术组(12只)。手术组通过缩窄左肾动脉及灌服附子汤建立肝阳上亢型肾性高血压大鼠模型。筛选模型成功大鼠50只,随机分为模型组(M组)、卡托普利组(C组)、风药组(W组)、天麻钩藤饮组(T组)、风药+天麻钩藤饮组(Z组),每组各10只,分别灌胃相应药物,1次/d;以等体积生理盐水灌胃正常组(N组)、假手术组(S组)与M组。试剂盒测定各组血清一氧化氮(NO)、内皮素(ET)、血栓素B2(TXB2)及6-酮-前列腺素(6-K-PGF1a)含量,免疫组化检测血管紧张素Ⅱ1型受体(AT1R)在大鼠胸主动脉中的表达。结果药物治疗4周后,M组的血压、血清ET、TXB2及胸主动脉AT1R表达较S组明显升高,而血清NO与6-K-PGF1a明显减低(P<0.05);W组与M组比较,上述指标无统计学意义(P>0.05);C组、T组及Z组的血压、血清ET、TXB2及胸主动脉AT1R表达较M组明显降低,而血清NO与6-K-PGF1a升高(P<0.05),其中Z组上述指标改善程度显著优于T组及C组(P<0.05),T组与C组比较,差异无统计学意义(P>0.05)。结论风药联合天麻钩藤饮对肝阳上亢型肾性高血压大鼠的降压效果优于单用天麻钩藤饮,风药可增强天麻钩藤饮的降压作用,其机制可能与降低血清ET、TXB2水平,抑制血管平滑肌AT1R表达,促进NO、6-K-PGF1a生成有关。
Objective To explore the antihypertensive and synergistic effect of wind medicine Fangfeng and Baizhi on renal hypertensive rats with syndrome of upper hyperactivity of liver yang and its mechanism.Methods One hundred male SD rats were randomly divided into normal group(n=12),operation group(n=76)and sham operation group(n=12).In the operation group,the model of renal hypertension was established by constricting the left renal artery and taking Fuzi decoction.Fifty successful rats were randomly divided into three groups:model group(group M),captopril group(group C),wind medicine group(group W),Tianma Gouteng decoction group(group T)and wind drug Tianma Gouteng decoction group(group Z),with 10 rats in each group.Normal group(group N),sham operation group(group S)and group M were perfused with normal saline of equal volume.The serum levels of nitric oxide(NO),endothelin(ET),thromboxane B 2(TXB 2)and 6-keto-prostaglandin(6-K-PGF1 a)were measured by Elisa and the expression of angiotensinⅡtype 1 receptor(AT1 R)in rat thoracic aorta was detected by immunohistochemistry.Results After 4 weeks of drug therapy,the blood pressure,serum ET,TXB 2 and AT1 R expression of thoracic aorta in group M were significantly higher than those in group S(P<0.05),but the expression of serum NO and 6-K-PGF1 a in group W were significantly lower than those in group S(P<0.05).There was no significant difference between group W and group M(P>0.05).The blood pressure,serum ET,TXB 2 and AT1 R expression of thoracic aorta in group C,group T and group Z were significantly lower than those in group M(P<0.05),but serum NO and 6-K-PGF1 a were higher than those in group M(P<0.05).The improvement of these indexes in group Z was significantly better than that in group T and group C(P<0.05),and there was no significant difference between group T and group C(P>0.05).Conclusion The hypotensive effect of wind medicine combined with Tianma Gouteng decoction on renal hypertensive rats with syndrome of upper hyperactivity of liver yang is better than that of Tianma Gouteng decoction alone.Wind medicine can enhance the antihypertensive effect of Tianma Gouteng decoction,and its mechanism may be related to the reduction of serum ET,TXB 2 level,inhibition of the AT1 R expression in vascular smooth muscle and the promotion of NO,6-K-PGF1 a formation.
作者
罗凤英
刘旺
LUO Fengying;LIU Wang(Zigong First People’s Hospital,Zigong 643099,China)
出处
《中西医结合研究》
2018年第2期75-79,共5页
Research of Integrated Traditional Chinese and Western Medicine
关键词
肝阳上亢型肾性高血压大鼠
血管活性物质
风药(防风、白芷)
降压增效作用
renal hypertensive rats with syndrome of upper hyperactivity of liver yang
vasoactive substances
wind drugs
Fangfeng
Baizhi
hypotensive and synergistic effect
