摘要
基因编辑技术是一种能够对生物体的基因组及其转录产物进行定点修饰或者修改的技术,早期基因编辑技术包括归巢内切酶、锌指核酸内切酶和类转录激活因子效应物。近年来,以CRISPR/Cas9系统为代表的新型技术使基因编辑的研究和应用领域得以迅速拓展。本文对基因编辑技术的原理、技术发展及其应用进行了阐述,对我国在基因编辑机制研究及技术发展、基因编辑动植物模型构建、基因治疗等领域的研究进展进行了回顾,并对基因技术的发展前景及趋势进行了展望。
Gene editing is a technology for precision modification of genome sequences or expressing transcripts.Early versions of such techniques include homing endonuclease,zinc finger endonuclease and transcription-like activator effectors.The CRISPR/Cas9 system has recently emerged as a powerful and versatile gene editing tool for a broad spectrum of applications.In this review,we discuss the recent development and applications of gene editing technology,and particularly focus on the progress in China,with the emphasis on mechanistic studies,development of new and extended technologies,the applications in animals,plants and the therapeutics.
作者
陈一欧
宝颖
马华峥
伊宗裔
周卓
魏文胜
Yiou Chen;Ying Bao;Huazheng Ma;Zongyi Yi;Zhuo Zhou;Wensheng Wei(School of Life Science,Peking University,Beijing 100871,China;Biomedical Pioneering Innovation Center,Peking University,Beijing 100871,China;Beijing Advanced Innovation Center of Genomics,Beijing 100871,China;PKU-Tsinghua Center for Life Sciences,Beijing 100871,China;Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China;Peking University Genome Editing Research Center,Beijing 100871,China)
出处
《遗传》
CAS
CSCD
北大核心
2018年第10期900-915,共16页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:31430025)资助~~
