摘要
为显著提高白腐菌(Irpex lacteus)产锰过氧化物酶(MnP)的活性。在液体培养条件下用分光光度计法检测12种诱导剂对乳白耙齿菌MnP活性的诱导效果。其中化学诱导剂包括愈创木酚、苯酚、香兰素、藜芦醇、苯甲酸、没食子酸、酪氨酸、二甲苯、苯胺蓝和ABTS,生物诱导剂包括麦麸和木屑。乳白耙齿菌在28℃下分别加入12种诱导剂进行培养,得到了不同时间的培养液,然后检测培养液中Mn P活性,从而得到乳白耙齿菌产MnP活性与诱导剂之间的关系。在初始培养基中加入0.2 mmol/L Mn^(2+)的培养条件下,诱导剂麦麸和ABTS对Mn P的诱导效果极为明显,二者在添加量分别为1.5%和0.2 mmol/L时,MnP活力分别为104.2 U/L和145.2 U/L,是初始产酶培养基酶活的8.6倍和11倍;而两种诱导剂组合,即同时添加1.5%麦麸与0.2 mmol/L ABTS,MnP活力为203.2 U/L,是初始培养条件MnP活性的21.9倍,实验表明诱导剂麦麸和ABTS能大幅度地提高白腐菌产MnP的活性。
In order to significantly increase the activity of Mn-P-producing bacteria(MnP)in white rot fungi Irpex lacteus,12 kinds of inducers were detected by spectrophotometry under liquid culture conditions.The chemical inducers were guaiacol,phenol,vanillin,resveratrol,benzoic acid,gallic acid,tyrosine,xylene,aniline blue,ABTS,and the bio-inducer were wheat bran,wood chips.The activity of MnP in the culture medium was measured by adding 12 kinds of inducers at 28℃,and the relationship between the activity of MnP and the inducer was obtained.0.2 mmol/L Mn 2+was added to the initial medium,and the effect of wheat bran and ABTS on MnP were obvious.The optimum additions were 1.5%and 0.2 mmol/L,respectively.Under these conditions the activity of MnP were 8.6 and 11 times that of the initial enzyme-producing medium.The two inducers were added with 1.5%of wheat bran and 0.2 mmol/L ABTS,the activity of the enzyme was 203.2 U/L,which was 21.9 times that under the initial culture condition.The results showed that the inducible wheat bran and ABTS could significantly increase the MnP activity of Irpex lacteus.
作者
何平
张健
池玉杰
HE Ping;ZHANG Jian;CHI Yu-jie(College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2018年第5期988-994,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
黑龙江省自然科学基金项目(C2016006)~~
关键词
白腐菌
乳白耙齿菌
诱导剂
锰过氧化物酶
酶活检测
white-rot fungi
Irpex lacteus
inducer
manganese peroxidase
enzyme activity detection
