摘要
建立BNL CL.2细胞氧化损伤模型,探讨岩藻黄素对细胞氧化损伤的保护作用。采用H2O2诱导,以MTT法筛选最佳诱导条件建立BNL CL.2氧化损伤模型。用不同浓度(1~20μmol/L)的岩藻黄素处理模型细胞,MTT法检测细胞活力,LDH试剂盒检测细胞乳酸脱氢酶释放量,GSH试剂盒检测胞内还原型谷胱甘肽含量,荧光酶标仪分析岩藻黄素对胞内活性氧(ROS)的清除能力。1 000μmol/L的H2O2处理BNL CL.2细胞24h成功建立了氧化损伤模型。20μmol/L岩藻黄素可显著提高模型细胞的活力(P <0.05);1~20μmol/L岩藻黄素可明显降低模型细胞乳酸脱氢酶释放量(P <0.05),可显著提高细胞内源性抗氧化剂谷胱甘肽的含量(P <0.05);1~10μmol/L岩藻黄素可显著降低模型细胞内ROS的含量(P <0.05)。岩藻黄素具有抗氧化作用,可抑制H2O2引起的BNL CL.2细胞的氧化损伤。
The aim of this study was to investigate the protective effect of fucoxanthin against H 2O 2-induced oxidative damage through establishing oxidative damage model of BNL CL.2 cells.BNL CL.2 cells were induced by H 2O 2 and MTT method was used to screen the appropriate concentration of H 2O 2 to establish oxidative damage model.BNL CL.2 cells were cultured in the medium supplemented with different concentration of fucoxanthin(1~20μmol/L).The effect of fucoxanthin on the cell viability was detected by MTT test.LDH release assay kit was used to detect the lactate dehydrogenase release of the cells.GSH assay kit was used to detect the GSH content of the cells.The reactive oxygen species(ROS)scavenging activity of FUCO was tested by fluorescence microplate reader.The model of oxidative damage in BNL CL.2 cells was successfully established by 1 000μmol/L H 2O 2 treatment for 24 h.Fucoxanthin at the concentration of 20μmol/L significantly increased cell viability of the oxidatively damaged cells(P<0.05).LDH Release of the H 2O 2-treated cells was significantly decreased(P<0.05),but the glutathione content was significantly increased by 1~20μmol/L fucoxanthin.The level of intracellular ROS was significantly decreased by 1~10μmol/L fucoxanthin(P<0.05).Fucoxanthin has antioxidant activity and can protect the BNL CL.2 cells from H 2O 2-induced oxidative damage.
作者
齐佳
崔艳君
王广策
唐娜
成敏
孙恒一
綦慧敏
刘顺梅
QI Jia;CUI Yan-Jun;WANG Guang-Ce;TANG Na;CHENG Min;SUN Heng-Yi;QI Hui-Min;LIU Shun-Mei(Weifang Medical University,Weifang 261053,China;Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第1期63-68,共6页
Periodical of Ocean University of China
基金
国家自然科学基金项目(31570941
81870237)
山东省自然科学基金项目(ZR2010DM010)
山东省医药卫生科技发展计划项目(2016WS0665
2016WS0672)
潍坊医学院大学生创新基金项目(KX2017007)资助~~
