摘要
目的探讨miR-16调节Bcl-2的表达活性影响胃癌细胞生物学行为的机制。方法 qPCR检测miR-16在胃癌SGC7901细胞中的转染效率,CCK-8检测miR-16对SGC7901细胞活性的影响,划痕实验检测miR-16对SGC7901细胞迁移能力的影响,流式细胞仪检测miR-16对SGC7901细胞凋亡的影响,Western blotting检测miR-16对Bcl-2蛋白表达的影响以及对凋亡相关蛋白表达的影响。结果通过miR-16mimic使miR-16在胃癌SGC7901细胞中过表达后,其细胞活性、迁移能力明显下降,而细胞凋亡率显著上升,同时相关凋亡蛋白表达也增高;Bcl-2siRNA与miR-16inhibitor共转染后,能改变miR-16对SGC7901细胞迁移能力、细胞活性和凋亡的影响。结论 miR-16通过调节Bcl-2的表达,抑制细胞活性和迁移能力,诱导胃癌SGC7901细胞凋亡。
Objective To explore the mechanism by which miR-16 regulates the expression of Bcl-2 on the biological behavior of gastric cancer cells.Methods qPCR was used to detect the transfection efficiency of miR-16 in gastric cancer SGC7901 cells.CCK-8 was used to detect the activity of SGC7901.Scratch assay was used to detect the effect of miR-16 on migration of SGC7901 cells.The effect of miR-16 on the apoptosis of SGC7901 cells was detected by flow cytometry.The effect of miR-16 on Bcl-2 protein expression was detected by Western blotting.Apoptosis-related proteins were detected by Western blotting.Results After miR-16 overexpressing in gastric cancer SGC7901 cells by miR-16 mimic,the cell viability and migration ability significantly decreased,while the apoptosis rate increased significantly.Cotransfection of Bcl-2 siRNA and miR-16 inhibitor changed the effect of miR-16 on the migration ability,cell viability and apoptosis of SGC7901 cells.Conclusion miR-16 can induce gastric cancer SGC7901 cell apoptosi,inhibit cell viability and migration ability by regulating the expression of Bcl-2.
作者
金向东
何旭华
叶盛威
刘洋
刘山
JIN Xiangdong;HE Xuhua;YE Shengwei;LIU Yang;LIU Shan(Department of Gastroenterology,Ezhou Third Hospital,Ezhou 436000,Hubei,China;Department of Gastrointestinal Surgery,Hubei Tumor Hospital,Wuhan 430000,China;Department of Thoracic Surgery,Wuhan Third Hospital,Wuhan 430000,China)
出处
《西部医学》
2018年第11期1573-1578,共6页
Medical Journal of West China
基金
湖北省自然科学基金(2011CDB312)
