摘要
为铁皮石斛原料药的生产提供一种新方法。以MS为基本培养基,探讨天然有机附加物与培养时间对铁皮石斛原球茎(PLBs)生长及主要药用成分石斛多糖和总生物碱的影响。研究结果表明:在添加不同浓度土豆汁的培养基中,以250 g/L效果最好,其干物率和多糖含量均在30天时达最大值,分别为8. 944%和17. 921%,增殖率在培养50天时最高,为1 028. 94%,而总生物碱含量在40天时积累最多,达0. 031 61%。增殖率、多糖和总生物碱含量均显著高于添加激素的对照组。添加番茄汁的培养基中各指标变化与土豆汁的类似,但原球茎长势差,颜色泛黄,增殖能力较添加土豆汁的差。综上,MS+250 g/L土豆汁+30 g/L蔗糖+7 g/L琼脂条有利于铁皮石斛PLBs生长和有效成分的积累,可考虑作为石斛多糖和石斛碱的生产来源,对铁皮石斛主要药用成分的生产具有重要的实践意义。
The aim of this paper was to provide a new way of the production of BPCs in Dendrobium officinale.Taking MS as minimal medium,adding nature organics,the effects on the growth of protocorm-like bodies(PLBs)from Dendrobium officinale and the major medicinal components including polysaccharide and total alkaloid were discussed in different incubation time.The results showed that the best concentration of potato juice was 250 g/L,the dry-weight percentage and polysaccharide contents both reached a maximum on the 30 days,which were 8.944%and 17.921%respectively,the highest proliferation rate was 1 028.94%on the 50 days,while the total alkaloid contents were accumulated on the 40 days,the maximum was 0.031 61%.The proliferation rate,polysaccharide and total alkaloid contents were significantly higher than the contrast with adding hormones.The variation of all indexes with adding tomato juice to the medium was similar to the potato juice,but the growth of PLBs was weak and the colour was yellow,the proliferative ability was poorer than adding potato juice.Taken together,the medium of MS+250 g/L potato juice+30 g/L sucrose+7 g/L agar was beneficial for the growth of PLBs from Dendrobium officinale and accumulating active ingredients,which could be considered as a polysaccharide and alkaloid production source,and it had very important practical significances to produce major medical ingredients of PLBs from Dendrobium officinale.
作者
肖小君
胡章薇
岳雨辰
何正兴
涂永燕
王辉
XIAO Xiao-jun;HU Zhang-wei;YUE Yu-chen;HE Zheng-xing;TU Yong-yan;WANG Hui(School of Life Science,Neijiang Normal University;Key Laboratory of Colleges and Universities in Sichuan for Research and Utilization of Distinctive Agricultural Undertakings,Neijiang,641100,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第11期1923-1927,1932,共6页
Natural Product Research and Development
基金
四川省科技厅项目(2015JY0242)
四川省教育厅自然科学研究项目(16ZB0304)
四川省教育厅科研创新团队(14TD0025)
内江师范学院校级科研项目(T160022)
关键词
天然有机物
铁皮石斛
原球茎
多糖
总生物碱
nature organics
Dedrobium officinale
protocorm-like bodies
polysaccharide
total alkaloid
