摘要
背景:单细胞纳米包裹技术是用极薄(<100nm)纳米膜包裹单个细胞的一项新技术。被纳米包裹的细胞已被广泛应用于医学生物学研究等各个领域。目的:综述单细胞纳米包裹技术的最新研究进展及应用,展望该技术未来的发展方向。方法:利用计算机在PubMed、CNKI、万方数据库中进行相关文献检索,中英文检索关键词为"single cell,nano-coating,nano-encapsulation,cell-in-shellstructure;单个细胞,纳米覆盖,纳米包裹",最终纳入52篇文献进行综述。结果与结论:多聚电解质的层层自组装技术具有过程简单、可调节,能形成性质多样的纳米膜等特征,已成为最广泛应用于合成单细胞外有机纳米膜的技术。应用多酚类物质的广泛黏附性及某些有机物能在细胞外自聚集的特性,也可形成性能较好的有机纳米包裹。除此之外,利用仿生矿化技术及利用某些无机物能直接在细胞表面结晶的性质,可在细胞表面形成无机纳米包裹。被纳米包裹的细胞已被广泛应用于单个细胞催化、细胞治疗、细胞保护、细胞生物学研究等各个领域。在未来的研究中,被包裹细胞的用途将更多地指导纳米膜材料的选择。
BACKGROUND:Single cell nanoencapsulation refers to coating single cells with ultrathin(<100 nm)layers and it has been widely used in medical and biological areas.OBJECTIVE:To review the development and applications of single cell nanoencapsulation.METHODS:The literature search was performed in PubMed,CNKI and WanFang database,and the keywords were“single cell,nano-coating,nano-encapsulation,cell-in-shell structure”in English and Chinese,respectively.According to the inclusion and exclusion criteria,52 articles were finally reviewed.RESULTS AND CONCLUSION:The layer-by-layer deposition technique is most widely used due to their versatile processing and tunable properties.And many other materials,like polyphenols and surface-induced hydrogelator have been also utilized as organic cell-coating materials.Besides,bioinspired mineralization and crystallization were utilized for creating inorganic nanoshells.Single cell nanoencapsulation has been widely used in many important areas,including cell biocatalysis,cell therapy,cytoprotection and cell-based biology.Extensive adhesion of polyphenols and self-assembling of some organic materials have been also utilized to form nanoencapsulation of single cells.Besides,bioinspired mineralization and crystallization have been utilized for creating inorganic nanoshells.Nanoencapsulated single cells have been widely used in many important areas,including cell biocatalysis,cell therapy,cytoprotection and cell-based biology.The application of encapsulated cells will make a more important part in the selection of nanocoating materials in future research.
作者
谢雯佳
王剑
裴锡波
Xie Wenjia;Wang Jian;Pei Xibo(Department of Prosthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610000,Sichuan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第2期278-283,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金青年基金(81601613
项目负责人:裴锡波)~~
