摘要
以木质素磺酸盐及其超滤分级分离产物为研究对象,系统考察了流动相pH值、离子强度等测试条件对超高效聚合物色谱(APC)法分析水溶性天然高分子相对分子质量的影响,并重点探讨了影响分析结果的非体积排阻效应。以pH6. 8的0. 1 mol/L NaNO3水溶液为流动相,采用3根Waters水溶性色谱柱串联,进样量为10μL,流速为0. 5 mL/min,木质素磺酸盐在色谱柱中获得了精细分离,其相对分子质量分布在8个相对分子质量区间。采用优化获得的测试条件表征木质素磺酸盐的超滤分级分离产物,测试结果表明,超滤法分离的3个组分实现了很好的色谱分离,对应的数均相对分子质量(Mn)分别为40 410、12 208和1 516 Da,证明超滤法对木质素磺酸盐实现了高效分离。该方法为木质素磺酸盐分级分离产物的进一步应用提供了测试基础。
The effects of testing conditions,such as pH and ionic strength of the mobile phase,on the relative molecular mass obtained by advanced polymer chromatography(APC)were investigated systematically.The non-size exclusion effects were discussed for lignosulfonate and its ultrafiltration fractionation product.The relative molecular mass distribution of lignosulfo-nate was well-characterized by an APC system using three columns in series,with a 10μL injection volume and 0.1 mol/L NaNO 3 as the aqueous mobile phase,at a flow rate of 0.5 mL/min.The relative molecular mass distribution is in the eight molecular mass ranges.Optimized test conditions were used to characterize the ultrafiltration fractionation product of lignosulfonate.The obtained number average relative molecular masses(M n)of the three components were 40 410,12 208,and 1 516 Da,indicating that lignosulfonate was well separated into three fractions by ultrafiltration membrane separation.The APC technique presented herein provides the basis for further application of the fractional separation products of lignosulfonate.
作者
钱晨
方红霞
蔡群
蔡方
高新宇
李丹丹
QIAN Chen;FANG Hongxia;CAI Qun;CAI Fang;GAO Xinyu;LI Dandan(Applied Chemistry Laboratory,Huangshan University,Huangshan 245041,China)
出处
《色谱》
CAS
CSCD
北大核心
2018年第12期1337-1341,共5页
Chinese Journal of Chromatography
基金
安徽省教育厅自然科学项目(KJHS2015B15)
安徽省省级大学生创新创业训练项目(201710375047)
黄山学院科研启动项目(2015XKJQ006)
安徽省创客实验室项目(2016ckjh190)~~
关键词
超高效聚合物色谱
水溶性高分子
木质素磺酸盐
超滤分级产物
advanced polymer chromatography
water soluble polymer
lignosulfonate
ultrafiltration fractionation product
