摘要
为了确定不同H_2O_2抑制剂对低温诱导植物花色素苷合成的影响,探讨此过程中不同来源H_2O_2的作用途径和机制,以四季秋海棠为研究试材,采用酶活性分析和qRT-PCR等方法,测定了不同处理下SOD、POD、PAO、GO酶的活性和H_2O_2、MDA的含量,以及花色素苷合成相关酶基因的表达水平。结果表明:低温显著增加了四季秋海棠叶片的SOD、POD、PAO、GO酶活性,促进了H_2O_2的积累和MDA含量的升高,最终引起花色素苷合成相关酶基因(BsPAL、BsCHS、BsF3H、BsANS)表达上调和花色素苷含量的增加;施用光合电子传递链的抑制剂DCMU、SOD的抑制剂DDC、GO酶的抑制剂OX和NADPH氧化酶的抑制剂DPI预处理6 h再给予低温处理,均不同程度地降低了SOD、POD、PAO、GO的活性和H_2O_2、MDA的含量,花色素苷合成相关酶基因表达水平下降,导致花色素苷合成受阻。因此,不同抑制剂预处理,分别降低由光合电子传递链、SOD、GO、NADPH氧化酶介导产生的H_2O_2后,均不同程度地减少了花色素苷含量,表明光合电子传递链、SOD、GO和NADPH氧化酶介导产生的H_2O_2可能参与了低温诱导四季秋海棠叶片花色素苷合成的过程。
To analyze the effects of different H 2 O 2 inhibitors on anthocyanin biosynthesis induced by low temperature,to discuss the function principle and function way of different originations of H 2O 2 produced in this process,the activity of SOD,POD,PAO and GO enzymes,the content of H 2O 2 and MDA,and the transcription levels of anthocyanin biosynthesis genes were measured under different treatments in Begonia semperflorens,using the methods of enzyme-activity analysis and qRT-PCR,etc..The results showed that:The activities of SOD,POD,PAO and GO were significantly increased by low temperature,causing H 2O 2 accumulation and an increase in MDA content in Beginia semperflorens.The transcription levels of anthocyanin biosynthesis genes(namely BsPAL,BsCHS,BsF 3 H and BsANS)and anthocyanin content were consequently soared in Beginia semperflorens;After the pretreatment with DCMU(an inhibitor of photosynthetic electron transport),DDC(an inhibitor of SOD),OX(an inhibitor of GO)and DPI(an inhibitor of NADPH oxidase)for 6 hours and then treated with low temperature,in different degrees,the activities of SOD,POD,PAO and GO all decreased,the contents of H 2O 2 and MDA decreased either;blocked partially anthocyanin biosynthesis by decreasing the transcription levels of anthocyanin biosynthesis genes.As a result,pretreatments of all the test inhibitors decreased the contents of H 2O 2 came from photosynthetic electron transport,SOD,GO and NADPH oxidase;and anthocyanin content was also decreased to different level in Beginia semperflorens.We therefore suggested that H 2O 2 produced by photosynthetic electron transport,SOD,GO and NADPH oxidase may be involved in low temperature induced anthocyanin biosynthesis in Beginia semperflorens.
作者
王阳
吴亚蓓
齐睿
田耕
白雪
李永华
张开明
WANG Yang;WU Yabei;QI Rui;TIAN Geng;BAI Xue;LI Yonghua;ZHANG Kaiming(College of Forestry,Henan Agricultural University,Zhengzhou 450002,China;Agricultural Economy and Information Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
出处
《河南农业科学》
CSCD
北大核心
2018年第11期110-115,共6页
Journal of Henan Agricultural Sciences
基金
河南省自然科学基金项目(182300410091)
关键词
四季秋海棠
花色素苷
低温
H2O2
诱导机理
Beginia semperflorens
Anthocyanin
Low temperature
H 2O 2
Induction mechanism
