摘要
小麦成熟期对粮食周年丰产具有重要的决定作用。为了给小麦分子标记辅助育种提供可用的分子标记,本研究以人工合成六倍体小麦(Bubo)和T.spelta L.衍生系(Turtur)为亲本创制的包含186个家系的RIL群体(F6)为材料,构建了包含5 301个标记(4 120个DArT标记、621个SNP标记和560个传统DArT标记),总长为2 464cM的遗传连锁图谱,利用Windows QTL Cartographer 2.5软件的复合区间作图法对在3年4点环境下的成熟期性状进行QTL检测,在LOD>2.5水平下,共定位到15个QTL,分布于小麦的1A、2B、2D、3A、4A、4B、5B、7A和7B染色体上,可解释4.42%～12.67%的表型变异。其中在1A染色体上控制小麦成熟期的QTL贡献率最大;4B染色体的1215714-1068877F0-44CG区间内3年3点均检测到的QTL与1215714标记遗传距离为0.01cM,近乎共分离,为下一步分子标记辅助选择的精准性提供了坚实的基础。
The maturity of wheat has an important role in determining the grain yield.In order to provide molecular marker for wheat molecular marker-assisted breeding,a population of 186 F 6 RILs derived from a cross between Triticum spelta L.(Turtur)and synthetic hexaploid wheat(Bubo)was used to detect the QTLs for maturity.A high-density genetic map was constructed,which includes 5301 markers(4 120 Silico DArT,621 SNPs and 560 traditional DArT array markers)with 2 464 cM.Using the field data in three years under four environments,QTLs associated with maturity were detected using composite interval mapping(CIM)method in Windows QTL Cartographer 2.5 software.Under the level of LOD>2.5,a total of 15 QTLs were detected on 1A,2B,2D,3A,4A,4B,5B,7A and 7B chromosomes.These QTLs could explain 4.42%-12.67%of phenotypic variance.Among them,the QTL on 1A chromosome has highest contribution rate.In the interval between 1215714 and 1068877F0-44CG on chromosome 4B,the QTL is only 0.01 cM away from 1215714 marker,which is almost co-separated.This study lays a solid foundation for the accuracy of molecular marker assisted selection.
作者
董爽爽
王利彬
程敦公
任勇
张业伦
穆平
张玉梅
刘建军
李豪圣
赵振东
郝元峰
曹新有
DONG Shuangshuang;WANG Libin;CHENG Dungong;REN Yong;ZHANG Yelun;MU Ping;ZHANG Yumei;LIU Jianjun;LI Haosheng;ZHAO Zhendong;HAO Yuanfeng;CAO Xinyou(College of Agronomy,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Laboratory for Wheat and Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley,Ministry of Agriculture,Jinan,Shandong 250100,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081 China;Mianyang Institute of Agricultural Sciences,Mianyang,Sichuan 621023,China;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei 050051,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2018年第11期1293-1299,共7页
Journal of Triticeae Crops
基金
国家重点研发计划项目(2016YFE0108600,2016YFD0101802)
山东省自然科学基金项目(ZR2016CQ33)
山东省农业科学院青年英才计划项目。
