摘要
背景:老年人群多同时患有骨性关节炎和骨质疏松症,但目前骨性关节炎与骨质疏松症两者之间的关系尚未明确。目的:构建骨性关节炎+骨质疏松症动物模型并进行鉴定。方法:贵阳医学院动物实验中心提供的37只2月龄雌性新西兰大白兔,30只随机分为骨性关节炎组、骨质疏松症组、骨性关节炎+骨质疏松症组,另设7只兔为正常对照组。分别制备骨质疏松症(造模方法:切除双侧卵巢+肌注甲泼尼龙)、骨性关节炎(造模方法:切除前交叉韧带)、骨性关节炎+骨质疏松症(造模方法:切除双侧卵巢+肌注甲泼尼龙+切除前交叉韧带)动物模型,确认造模成功后对上述3种模型软骨进行分离及培养,比较3者软骨细胞中Ⅱ型胶原酶分泌情况。结果与结论:①运用切除兔前交叉韧带、卵巢及肌注甲泼尼松龙琥珀酸钠的方法可使骨质疏松兔骨密度降低25%以上,从关节外观可见明显关节软骨剥脱,膝关节X射线检查可见明显骨赘生成,MRI检测可显示明显关节软骨损伤,由此可知通过上述方法可成功制备骨质疏松症合并骨性关节炎的模型;②骨性关节炎组、骨质疏松症组、骨性关节炎+骨质疏松症3组兔膝关节软骨中均能成功提取出软骨细胞,与骨质疏松症组比较骨性关节炎+骨质疏松组软骨细胞Ⅱ型胶原酶的能力下降差异明显(P<0.05);③结果说明:实验成功建立了骨性关节炎+骨质疏松症动物模型。
BACKGROUND:The elderly often suffers from comorbid osteoarthritis and osteoporosis,but the relationship between osteoarthritis and osteoporosis is not yet clear.OBJECTIVE:To establish and identify an animal model of comorbid osteoarthritis and osteoporosis.METHODS:Thirty-seven female New Zealand white rabbits at 2 months of age were provided by the Animal Center of Guiyang Medical University,China.Of the 37 rabbits,30 rabbits were selected and divided into osteoarthritis group,osteoporosis group,osteoarthritis and osteoporosis group.The remaining seven rabbits were used as normal control group.Different animal models of osteoporosis(excision of bilateral ovarian and intramuscular injection of methylprednisolone)/osteoarthritis(excision of anterior cruciate ligament)/osteoarthritis and osteoporosis(excision of bilateral ovaries and anterior cruciate ligament+intramuscular injection of methylprednisolone)were made in corresponding groups.After successful modeling,cartilage samples from each rabbit were isolated and cultured.Secretion of type II collagenase was compared among three groups.RESULTS AND CONCLUSION:(1)After excision of rabbit anterior cruciate ligament and bilateral ovaries and intramuscular injection of methylprednisolone,the bone density of osteporotic rabbits was reduced by more than 25%.Exfoliation of the articular cartilage could be observed from the joint appearance,and osteophyte formed markedly shown on the X-ray film of the knee joint obviously.Obvious articular cartilage damage was also observed on the MRI image.Therefore,the model of comorbid osteoarthritis and osteoporosis was successfully prepared by the above methods.(2)Chondrocytes could be successfully extracted from the rabbit knee joint cartilage in the three model groups.The ability of chondrocytes secreting type II collagenase was significantly lowered in the osteoporosis group compared to the osteoarthritis and osteoporosis group(P<0.05).(3)To conclude,the animal model of comorbid osteoarthritis and osteoporosis was successfully established.
作者
马文娟
张开伟
沈冯君
邓致远
程效进
Ma Wenjuan;Zhang Kaiwei;Shen Fengjun;Deng Zhiyuan;Cheng Xiaojin(Jinsha Country Hospital of Traditional Chinese Medicine,Jinsha 551800,Guizhou Province,China;Guizhou Provincial Hospital of Traditional Chinese Medicine,Guiyang 550000,Guizhou Province,China;Department of Pain,Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430050,Hubei Province,China;Caoxian Country Hospital of Traditional Chinese Medicine,Caoxian 274400,Shandong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第3期421-426,共6页
Chinese Journal of Tissue Engineering Research
基金
贵州省优秀科技教育人才省长专项资金(黔省专合字[2012]34号)
项目负责人:张开伟
贵州省科技厅项目(黔科合中药字[2011]LKZ7022号)
项目负责人:张开伟~~
