摘要
目的观察梅毒血清固定患者外周血单核细胞中Toll样受体2-mRNA(TLR2-mRNA)的表达,并分析其临床意义。方法选取2016年5月-2017年5月在我院门诊接受观察/随访的梅毒血清固定患者为观察对象,同时选取同期在我院体检的健康成年人作为对照,观察两组Toll样受体2-mRNA在外周血单核细胞中的表达水平,比较不同临床特征梅毒患者外周血单核细胞中Toll样受体2-mRNA表达情况。结果梅毒血清固定患者外周血单核细胞中TLR2-mRNA表达水平低于对照组(t=11.205,P<0.001);病情程度为晚期潜伏梅毒、病程≥3年的梅毒血清固定患者外周血单核细胞中TLR2-mRNA表达水平较低,且不同年龄、性别的患者外周血单核细胞中TLR2-mRNA表达水平无明显差别;病情程度是影响梅毒血清固定患者外周血单核细胞中TLR2-mRNA表达水平的因素。结论梅毒血清固定患者外周血单核细胞中Toll样受体2-mRNA表达水平较低,且与病情程度密切相关。
Objective To observe the expression of Toll like receptor 2(TLR 2)mRNA in peripheral blood mononuclear cells of patients with syphilis and its clinical significance.Methods The patients with syphilis serum fixed in Our hospital outpatient treatment from May 2016 to May 2017 were included,and the healthy adults who were examined in our hospital during the same period were selected as control,The mRNA expression of TLR 2 in peripheral blood mononuclear cells(PBMCs)in two groups were observed.The mRNA expression of TLR 2 in PBMCs of patients with different clinical features was compared.Results The expression level of TLR 2-mRNA in PBMCs of patients with syphilis was lower than that in control group(t=11.205,P<0.001);The TLR 2-mRNA expression level of serofast patients with severity of late latent syphilis,course more than 3 years in PBMCs is low,and the TLR 2-mRNA of different age and sex of patients with PBMCs had no significant difference in expression level;Degree of illness of TLR 2-mRNA in PBMCs of patients with syphilis.Conclusion The expression of Toll like receptor 2-mRNA in PBMCs of patients with syphilis is low,and it is closely related with degree of illness.
作者
李燕琼
张智敏
LI Yanqiong;ZHANG Zhimin(Department of blood transfusion,Zhumadian Central Hospital,Zhumadian,Henan 463000,China;epartment of clinical laboratory,Zhumadian Central Hospital,Zhumadian,Henan 463000,China)
出处
《实验与检验医学》
CAS
2018年第6期856-858,913,共4页
Experimental and Laboratory Medicine
