摘要
分别以菌丝生长速度和麦角甾醇含量为指标,采用单因素和正交实验优化赭鳞蘑菇(Agaricus subrufescens)固体发酵培养基,用优化后的固体发酵培养基培养,测定期间菌质干重及麦角甾醇含量,判定发酵终点。结果表明:赭鳞蘑菇最优固体发酵培养基为50 g玉米糁添加50 mL含35 g/L白砂糖和80 g/L麸皮(煮汁)的营养液,最佳发酵终点为培养40 d,此时菌质干重为91.45 g,麦角甾醇总量为31.426 mg。
Culture medium for solid-state fermentation of Agaricus subrufescens was optimized by the single-factor and orthogonal experimental design using mycelial growth rate and ergosterol content as indicators.Dry fungal substance weight and ergosterol content were monitored during the60-day fermentation to determine the end point of fermentation.The results showed that the optimal medium for solid-state fermentation of A.subrufescens was50g corn grit as the base material with addition of50mL nutrient solution containing35g/L white granulated sugar and80g/L wheat bran(boiled and collected the filtrate).The optimal fermentation period was determined to be40days,when the dry fungal substance weight and the total ergosterol were91.45g and31.426mg,respectively.
作者
范秀芝
殷朝敏
金玮玲
叶罗娜
史德芳
姚芬
高虹
FAN Xiuzhi;YIN Chaomin;JIN Weiling;YE Luona;SHI Defang;YAO Fen;GAO Hong(Institute of Agro-Products Processing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan,Hubei430064,China)
出处
《食用菌学报》
CSCD
北大核心
2018年第4期35-42,共8页
Acta Edulis Fungi
基金
湖北省农业科学院竞争性计划项目(2016jzxjh016)
国家自然科学基金(31601806)
关键词
赭鳞蘑菇
固体发酵
菌质
菌丝生长速度
麦角甾醇
Agaricus subrufescens
solid-state fermentation
fungal substrate
mycelial growth rate
ergosterol
