摘要
对开阳县某蛋鸡场发生的疫病通过发病情况调查、剖检病变观察、细菌分离培养和相关病毒PCR检测。结果:疫病主要发生于160日龄以上鸡群,病变以肝脏和脾脏显著肿大为特征;细菌分离培养未见菌落生长; PCR检测可见禽白血病病毒特异性DNA片段,但未检出鸡马立克氏病病毒特异性DNA片段。综合诊断为禽白血病病毒感染。
The dead chicken cases,occurred in a laying hen farm in Kaiyang county,were diagnosed by incidence investigation?observation of diseased lesion,isolation and culture of bacteria and PCR detection of virus nucleic acid.Results:this cases mainly occurred in chickens over160days old,and the dissection lesion were characterized by marked enlargement of liver and spleen in the sick chickens.No bacterial growth were observed after isolation and culture.The specific DNA fragments of ALV were detected by RT-PCR in liver of the sick chickens,but the fragments of MDV were not detected.These results suggested that this case was caused by avian leukosis virus infection.
作者
晏晓洪
胡安东
贺欣薇
温贵兰
文明
皮泉
张霞
Yan Xiaohong;Hu Andong;He Xinwei;Wen Guilan;Wen Ming;Pi Quan;Zhang Xia(College of Animal Science,Guizhou University,Guiyang Guizhou550025,China;Guizhou Centre for Prevention and Control of Animal Disease,Guiyang Guizhou550008,China)
出处
《贵州畜牧兽医》
2018年第6期26-28,共3页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
基金
"贵州省家禽疫病监测与控制"贵州省农业委员会兽医科技项目(黔农合字[2018]0704号)
关键词
禽白血病
细菌分离培养
病毒PCR检测
Avian Leukosis
Isolation and Culture of Bacteria
PCR Detection of Virus
