摘要
目的]印度黄檀叶含有多酚及类黄酮物质,研究印度黄檀叶多酚及其抗氧化活性,可为其利用提供依据。[方法]以印度黄檀叶为原料,乙醇为提取液,经单因素实验与正交试验设计,检测在不同乙醇浓度、提取时间、提取温度及超声功率120 w时3个因素进行响应面优化试验,确定印度黄檀多酚的提取工艺;同时,鉴定印度黄檀叶乙醇-水提取液对DPPH-自由基的清除能力。[结果]低浓度印度黄檀叶多酚能发挥更强的抗氧化能力,其提取液对清除DPPH自由基的半数抑制质量浓度(IC_(50))约为3.2 mg·L^(-1),略大于Vc的2.5 mg·L^(-1);不过,其还原能力略低于Vc。[结论]印度黄檀叶内富含多酚类物质,具有很强的体外抗氧化活性,可作为天然抗氧化植物资源开发利用。
[Objective]The leaves of Dalbergia sissoo(Roxb.)contain polyphenols and flavonoids.Polyphenols and flavonoids are scavengers of free radicals in organisms,preventing the toxic effects of free radicals on the body and inhibiting lipid peroxidation,i.e.antioxidant activity.Therefore,understanding of polyphenols extraction form leaves and its antioxidant activity in vitro could provide theoretical basis of utilization of leaves of D.sissoo.[Method]Ethanol was used as the extract of polyphenols in leaves of D.sissoo,and then single factor experiment and orthogonal experiment were used to determine the extraction process of polyphenols.At the same time,the removal capacity of DPPH-free radical of ethanol-water extract of leaves of D.sissoo was identified.[Result]Low-concentration of polyphenol solution of leaves of D.sissoo had strong antioxidant activity.The concentration of polyphenol solution for inhibitory half number of DPPH free radical(IC 50)was about 3.2 mg·L^-1,much higher than concentration 2.5 mg·L^-1 of Vc,but its reducing ability was slightly lower than the Vc.[Conclusion]Leaves of D.sissoo are rich in polyphenols and have strong antioxidant activity,which can be used as natural antioxidant plant resources for development and utilization.
作者
邓疆
王有琼
石雷
DENG Jiang;WANG You-qiong;SHI Lei(Research Institute of Resources Insects,Chinese Academy of Forestry,Kunming 650224,Yunnan,China)
出处
《林业科学研究》
CSCD
北大核心
2018年第6期161-167,共7页
Forest Research
基金
国家重点研发计划项目子课题资助:印度黄檀高效培育技术研究(2016YFD0600605)
关键词
印度黄檀
叶
多酚
微波萃取
抗氧化活性
Dalbergia sissoo
polyphenols
microwave extraction
antioxidant activity
