摘要
为进一步明确Sy11、W-7、SR13-2、ST-1和HT-6这5株拮抗菌复合发酵得到的菌液(CFBF-Ⅱ)对马铃薯的防病促生潜力,本试验采用浸泡、浇灌等方法探讨了CFBF-Ⅱ对马铃薯种薯萌发、植株生长、离体叶片防病和根系微生物群落的影响。结果显示,CFBF-Ⅱ处理后种薯芽高、芽直径和萌发率均明显优于4株拮抗菌(Sy11、SR13-2、ST-1和HT-6)的复合发酵菌液(CFBF-Ⅰ)以及单一菌株HT-6菌液的处理;CFBF-Ⅱ在离体叶片上对晚疫病的预防效果也最好,达到94.6%,显著优于HT-6和甲霜灵锰锌处理;叶片中叶绿素尤其是叶绿素总含量以及叶绿素b的含量以CFBF-Ⅱ处理最高,与甲霜灵锰锌处理和LB对照之间均达到显著性差异(P<0.05);植株生长至第55天,CFBF-Ⅱ处理的株高、茎粗、叶片数和叶面积比LB对照分别增加了14.8%,26.5%,16.6%和32.9%,也明显优于HT-6的促生效果。此外,CFBF-Ⅱ处理后第50天,马铃薯根际土壤中细菌数量与对照基本一致,但显著高于甲霜灵锰锌处理,真菌数量显著低于甲霜灵锰锌处理和对照,放线菌数量均与甲霜灵锰锌处理相近但均显著低于对照。以上结果表明这5株菌之间的相互作用不仅能更好地直接抑制致病疫霉生长、预防马铃薯晚疫病并促进植株生长,还能改善土壤微生物区系的群落结构和功能。
In order to further clarify the integrated potential of compound fermentation bacterial fluid(CFBF-Ⅱ)of Sy11,W-7,SR13-2,ST-1 and HT-6 against potato late blight and promotion for potato plant growth,the soaking and root-irrigation methods were adopted for exploring the effects of CFBF-Ⅱon seed tube germination,plant growth,in vitro leaf disease prevention and rhizosphere microbial community.The results showed that the seed bud height,bud diameter and germination rate of CFBF-Ⅱtreatment were significantly higher than that of CFBF-Ⅰ(Sy11,SR13-2,ST-1 and HT-6)and single strain HT-6 treatments.Compared with HT-6 and metalaxyl Mn-Zn treatments,CFBF-Ⅱhad the best prevention effect(94.6%)against late blight on potato leaves.The content of chlorophyll b and the total content of chlorophyll in potato leaves were the highest with CFBF-Ⅱ,which was significantly different from that treated with metalaxyl Mn-Zn and LB control(P<0.05).On the 55 th day after seedling emergence,the plant height,stem diameter,leaf number and leaf area of CFBF-Ⅱtreatment increased by 14.8%,26.5%,16.6%and 32.9%,respectively,in comparison with LB control,which were also better than the promoting effect of HT-6.In addition,on the 50 th day after CFBF-Ⅱtreatment,the amount of bacteria in potato rhizosphere soil was similar to that of LB control,but significantly higher than that of metalaxyl Mn-Zn treatment.The number of fungi was significantly lower than that of metalaxyl Mn-Zn and LB.The number of actinomycetes were similar to that of metalaxyl Mn-Zn,but lower than that of LB control.The these results suggest that the interaction between the five antagonistic strains not only can better inhibit the growth of Phytophthora infestans,control potato late blight and promote plant growth,but also improve the community structure and function of soil microflora.
作者
申芬
蒋继志
侯宁
李成斌
田荟遥
李岩
SHEN Fen;JIANG Ji-zhi;HOU Ning;LI Cheng-bin;TIAN Hui-yao;LI Yan(College of Life Sciences,Hebei University,Baoding 071002,China;Department of Basic Medicine, Xingtai Medical College,Xingtai 054000,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2018年第6期69-74,共6页
Journal of Hebei Agricultural University
基金
河北省自然科学基金项目(C 2015201231)
公益性行业(农业)科研专项(201303018)
关键词
复合发酵
致病疫霉
离体防病
促生作用
根系微生物
compound fermentation
Phytophthora infestans
in vitro disease prevention
promoting effect
root microorganism
