摘要
在丙酸杆菌(Propionibacterium freudenreichii)厌氧发酵生产脱氧腺苷钴胺素(deoxyadenosylcobalamin,简称ADO)的过程中,中间体腺苷钴啉醇酰胺(adenosylcobinamide,简称CBI)和5,6-二甲基苯并咪唑(DMB)结合后生成产物ADO。目前维生素B12脱氧腺苷钴胺素发酵过程中的实时在线检测方法相对匮乏,为了解决该问题,提高维生素B12的发酵单位,以CBI为目标,运用代谢调控方法改善DMB的加入时间,研究不同时间加入DMB对丙酸杆菌生长以及ADO合成的影响。在分批次发酵情况下,实时检测了菌体发酵过程中具体的CBI合成量、ADO产量,并进一步绘制了菌体的生长曲线。结果表明,DMB对丙酸杆菌生长的抑制作用并不明显,但是可以抑制单位菌体产量,发酵周期为120h,最佳添加时间为90～100h,相比60h添加DMB可提高发酵单位59.08%。方法简便、快捷、高效,对维生素B12的发酵调控研究具有一定的参考价值。
In the process of anaerobic fermentation of Propionibacterium freudenreichii to produce vitamin B 12 deoxyadenosylcobalamin(ADO),the intermediate adenosylcobinamide(CBI)and 5,6-dimethylbenzimidazole(DMB)are combined to produce the product ADO.At present,there is lack of detection means for vitamin B 12.To solve the problem,taking CBI as the research goal,the metabolic regulation method is used to improve the addition time of DMB,and the effects of different DMB adding time on the growth of bacteria and ADO synthesis are studied.Under the conditions of batch fermentation,the CBI synthesis amount and ADO yield of Propionibacterium are determined,and the growth curve of the bacteria is drawn.It is found that the inhibitory effect of DMB on the growth of the cells is not obvious,but the unit cell yield could be inhibited.The fermentation cycle is 120 h,and the optimal addition time is 90~100 h.Compared with 60 h,the addition of DMB can increase the fermentation unit by 59.08%.The method is simple,rapid,efficient,and has a certain guiding effect on the study of fermentation regulation of vitamin B 12.
作者
王鹏
李路伟
郭金凤
徐凯莉
董哲
WANG Peng;LI Luwei;GUO Jinfeng;XU Kaili;DONG Zhe(School of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei050018,China)
出处
《河北科技大学学报》
CAS
2018年第6期546-551,共6页
Journal of Hebei University of Science and Technology
基金
河北省食品药品监督管理局科技计划项目(QN2015017)
河北省科技计划项目(16273812)
河北省高等学校科学技术研究项目(BJ2017059)
