摘要
为了探究猪缝隙连接蛋白43(GJA1)的结构和相关功能,本研究对16个哺乳动物的GJA1蛋白进行多重序列比对及系统发育分析,并通过相关的生物信息学数据库和软件从理化性质、蛋白修饰位点、跨膜区、亚细胞定位、二级结构、三级结构、多态位点、功能区域和互作蛋白等方面对猪GJA1蛋白的结构和功能进行系统分析。系统发育分析结果显示,GJA1蛋白在16种哺乳动物中变异未达到饱和,物种间遗传距离小且同源性高,其中猪与马的GJA1蛋白序列最为接近,同源性达93.3%,在进化树上的分类也证实了这一结果。蛋白结构分析结果显示,猪GJA1蛋白分子质量为43.08ku,理论等电点为8.88,由20种氨基酸组成,不稳定指数为44.06,平均疏水系数为-0.240,脂溶指数为82.67,不分泌信号肽,有4个跨膜区、6个O-糖基化位点和38个磷酸化位点,最有可能位于细胞质膜和高尔基体内,其二级结构和三级结构主要由α-螺旋和无规则卷曲组成。蛋白功能分析结果显示,在猪GJA1蛋白的CDS序列中有2个超级家族结构功能区域和6个错义突变位点,且猪GJA1蛋白还与ZO-1和Cx36等蛋白及多种蛋白激酶存在相互作用。本研究通过生物信息学分析系统地揭示了猪GJA1蛋白的结构和功能,为后续进一步研究GJA1蛋白在猪体内发挥生物学功能的遗传调控机制提供一定的理论支持。
To explore the structure and function of porcine connexin 43(GJA1)protein,this study compared the coding sequences of GJA1 protein in 16 different species to analyze evolution and phylogeny relationship.The amino acid sequence information of porcine GJA1 protein such as physical and chemical properties,protein modification sites,transmembrane regions,subcellular localization,secondary and tertiary structures,polymorphic sites,functional regions and interaction proteins were analyzed by the related bioinformatic softwares and databases.Phylogeneticanalysis results showed the variation of GJA1 protein did not reach saturation,the protein sequence displayed small genetic distance and high homology among 16 species.The porcine GJA1 protein had the greatest genetic relationship with that of equine,and the classification in the evolutionary tree also confirmed it.The protein structure analysis results indicated that the porcine GJA1 protein was composed of 20 kinds of amino acids with the molecular weight of 43.08 ku and theoretical isoelectric point of 8.88.It was predicted that the instability index of porcine GJA1 protein was 44.06,the lipid solubility index was 82.67,and its hydrophobic average coefficient was-0.240.There were 4 transmembrane regions,6 O-GlcNAc sites,38 phosphorylation sites and no signal peptide.The GJA1 protein was most likely exist in the plasma membrane and the Golgi apparatus,and its secondary and tertiary structures were mainly comprised ofα-helix and random coil.The protein function analysis results demonstrated that the porcine GJA1 protein had2 superfamily functional regions and 6 missense mutation sites in its CDS sequence.In addition,the porcine GJA1 protein interacted with ZO-1,Cx36 and many protein kinases.This study systematically revealed the structure and function of porcine GJA1 protein by bioinformatics analysis,which could provide a theoretical reference for further researches on the genetic regulatory mechanism of GJA1 protein in pigs.
作者
戴开宇
曹越
郜重丞
王海飞
包文斌
吴圣龙
DAI Kaiyu;CAO Yue;GAO Zhongcheng;WANG Haifei;BAO Wenbin;WU Shenglong(Key Laboratory for Animal Genetics ,Breeding,Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology ,Yangzhou University, Yangzhou 225009,China;Seed-breeding and Innovation Team of Jiangsu Province Modern Agriculture (Szvine) Industry Technical System,Yangzhou 225009, China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第12期3305-3316,共12页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31702082、31772560)
扬大青蓝工程和和扬州大学中青年学术带头人
