摘要
2016年8月,于湖南张家界武陵源黄龙洞捕获一种鱼类,对其10尾标本进行形态学观察、测量与计数,确定为野鲮亚科异华鲮属鱼类。采用PCR技术对其线粒体细胞色素b基因进行扩增,测定其基因序列,并与美国国立生物技术信息中心数据库对比,发现其与长鳍异华鲮(Parasinilabeo longiventralis)、长体异华鲮(Parasinilabeo longicorpus)、异华鲮(Parasinilabeo assimilis)的遗传距离分别为0.5%、1.6%~1.8%、1.9%;将其形态数据与以上3种鱼的形态原始测量数据进行对比,发现其与长鳍异华鲮在形态学上最接近,但其个体偏小,大部分数据交叉,各鳍条数少1~2个。初步确定该鱼类为长鳍异华鲮,是湖南省的新记录种。对该种鱼的分布区域、生存环境的初步分析认为,长鳍异华鲮是栖息在亚热带季风湿润气候带的喀斯特地区的季节性洞穴鱼类。
In August of2016,one kind of fish was collected at Huanglong Cave,Wulingyuan,Zhangjiajie,Hunan Province.The detailed morphology of the10specimens were observed,measured and counted and then they were identified as a Parasinilabeo fish subordinating to Labeoninae.The mitochondrial cytochrome b gene was amplified with PCR technology and the sequenced were compared with the National Center for Biotechnology Information Database,the United States.The results showed that the genetic distance between the studied species and Parasinilabeo longiventralis,Parasinilabeo longicorpus and Parasinilabeo assimilis were0.5%,1.6%-1.8%and1.9%,respectively.Compared with the above-mentioned three kinds of fishes in morphology,it was closest to Parasinilabeo longiventralis,but owing smaller individual sizes and less number of fin by1-2.According to the molecular and morphological data,it was identified as Parasinilabeo longiventralis,which was a new record in Hunan province.After preliminary analysis of the distribution area and living environment,the Parasinilabeo longiventralis should be a seasonal cave fish that inhabits the karst region in the subtropical monsoon wet climate zone.
作者
罗庆华
胡骁
陶水秀
陈进豪
刘柯
LUO Qinghua;HU Xiao;TAO Shuixiu;CHEN Jinhao;LIU Ke(School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083,China;Hunan Engineering Laboratory for Chinese Giant Salamander’s Resource Protection and ComprehensiveUtilization, Zhangjiajie, Hunan 427000, China;College of Biology and Environmental Sciences, Jishou University,Jishou, Hunan 416000, China;School of Civil Engineering and Architecture, Jishou University, Zhangjiajie, Hunan427000, China)
出处
《湖南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2018年第6期650-654,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(31460160)
大鲵工程实验室开放项目(DNGC1705)
关键词
长鳍异华鲮
新记录种
形态特征
线粒体细胞色素B基因
湖南
Parasinilabeo longiventralis
new record species
morphological characteristics
mitochondrial Cyt b gene
Hunan