摘要
目的通过研究肝泡型包虫病(HAE)患者外周血B淋巴细胞部分表面抗原的表达及所分泌的抗体水平变化,探讨肝包虫病患者体液免疫的变化情况。方法用10μL CD19-FITC、10μL CD20-PE标记100μL外周血中B淋巴细胞,经避光反应、裂红、洗涤等步骤后用流式细胞仪测定CD19、CD20的表达,并用蛋白分析仪检测IgG、IgM、IgA的表达水平。结果 HAE患者外周血CD19^+、CD20^+B淋巴细胞比率均低于正常对照组,差异有统计学意义(P<0.01)。HAE组IgG、IgA水平均明显高于对照组,IgM水平低于对照组,差异均有统计学意义(P<0.05)。结论泡球蚴在体内寄生过程中,宿主的免疫反应处于抑制状态,使得泡球蚴能在宿主内长期寄生并发育,同时能刺激机体产生强弱不等的免疫应答反应。
Objective To investigate the change of humoral immune in the patients with hepatic alveolar echinococcosis(HAE)by studying the expression of partial surface antigens and excreted antibody level change of B lymphocyte in peripheral blood.Methods The B cells in 100μL peripheral blood were labeled by 10μL CD19-FITC and 10μL CD20-PE,and then the expressions of CD19 and CD20 were detected by flow cytometry after lightless reaction,lyticing red blood cell and washing.The expression levels of IgG,IgM and IgA were detected by the protein analyzer.Results The ratio of CD19^+and CD20^+cells in peripheral blood of HAE patients was lower than that of the normal control group,and the difference was statistically significant(P<0.01).The levels of IgG and IgA in the HAE group were significantly higher than those in the control group,the level of IgM was lower than that in the control group,and the differences were statistically significant(P<0.05).Conclusion During the in vivo parasitic process of alveolar echinococcosis,the host′s immune response is in inhibitory status,which enables alveolar echinococcosis to parasitize and develop in the host for a long time,meanwhile stimulates the body to produce varying intensity degrees of immune response.
作者
马婕
冀林华
刘文静
崔森
樊海宁
MA Jie;JI Linhua;LIU Wenjing;CUI Sen;FAN Haining(Affiliated Hospital of Qinghai University,Xining,Qinghai 810001,China;Research Center for High Altitude Medicine,Qinghai University,Xining,Qinghai 810001,China)
出处
《重庆医学》
CAS
2018年第35期4479-4481,4486,共4页
Chongqing medicine
基金
国家人力资源与社会保障部2017年高层次留学回国人才资助项目(2017-200)
青海省科技厅项目(2014-ZJ-719)
青海大学附属医院中青年科研基金项目(ASFR-2015-YB)
关键词
棘球蚴病
肝
B淋巴细胞
体液免疫
echinococcosis,hepatic
B lymphocytes
humoral immunity
