摘要
目的探讨抑癌基因PRDM5表达下降是否是HPV16病毒感染导致宫颈癌发生的机制之一。方法采用免疫组化法检测宫颈癌组织及正常宫颈组织中PRDM5蛋白及HPV16 E6/HPV18 E6蛋白的表达; HPV16 E6 shRNA质粒转染Si Ha细胞后,应用RT-PCR及Western blot法检测PRDM5的表达。结果 HPV16/18 E6在宫颈癌组织中表达阳性率明显高于正常宫颈组织;宫颈癌组织中PRDM5蛋白表达水平较正常宫颈组织中表达水平低;将HPV16 E6 shRNA3转染SiHa细胞干扰HPV16 E6表达后,发现Si Ha细胞中PRDM5 mRNA及蛋白表达上调。结论 PRDM5可能介导HPV16病毒感染导致宫颈癌的发生发展过程。
Objective The aim of this study was to explore whether the down-regulation of tumor suppressor gene PRDM5 is one of the mechanisms of HPV16 virus infection leading to cervical cancer.Methods The expressions of PRDM5 protein and HPV16 E6/HPV18 E6 protein in cervical cancer tissues and normal cervical tissues were detected by immunohistochemistry.After transfected with HPV16 E6 shRNA plasmid,the expression of PRDM5 gene was detected in SiHa cells by RT-PCR and Western blot.Results The positive expression rate of HPV 16/18 E6 in cervical cancer tissues was significantly higher than that in normal cervical tissues.The expression level of PRDM5 protein in cervical cancer tissues was lower than that in normal cervical tissues.After HPV16 E6 shRNA3 was transfected into SiHa cells to interfere with the expression of HPV16 E6 gene,the expression of PRDM5 at mRNA and protein levels was up-regulated in SiHa cells.Conclusion PRDM5 may mediate the development of cervical cancer caused by HPV16 virus infection.
作者
成海燕
马晶
冯紫微
黄可金
王玉霞
张云艳
CHENG Haiyan;MA Jing;FENG Ziwei;HUANG Kejin;WANG Yuxia;ZHANG Yunyan(Harbin Medical University Cancer Hospital,Harbin 150081,China)
出处
《实用肿瘤学杂志》
CAS
2018年第6期483-487,共5页
Practical Oncology Journal
基金
国家自然科学基金(编号:8167102096)
黑龙江省青年科学基金项目(编号:QC2014C105)
黑龙江省卫生厅课题(编号:2011-129)
黑龙江省肿瘤医院院内科研启动资金(编号:JJ2010-22)
