摘要
为提高杨木纤维的糖化利用率,采用酸处理的方式制备杨木纤维还原糖,对硫酸浓度、水解时间和液料比分别进行单因素试验,根据单因素实验结果设计Box-Behnken中心组合试验,以还原糖提取率为指标值,采用响应面分析法确定降解的最优工艺参数。并以混合培养纤维素生产菌的方式提高纤维素酶的生产量酶解杨木纤维产还原糖。结果表明:酸预处理过程产还原糖最优化条件为,液料比10.03、酸浓度2.03%、水解时间103 min,还原糖提取率平均值为19.31%。获得能大幅度提升纤维素酶活的菌种比例为斜卧青霉和里氏木霉RutC-30,接种比例为3∶7,酶解得率为21.7%。为后续的木质纤维素应用转化为其它产物提供了支撑基础。
In order to improve the saccharification utilization rate of poplar fiber,poplar fiber reducing sugar was prepared by acid treatment.And the single factor experiments were conducted on the concentration of sulfuric acid,the hydrolysis time and the ratio of solid to liquid,which the Box-Behnken center combination test was designed based on the single factor experimental results.With the reducing sugar content as the index value,the response surface methodology was used to determine the optimal process parameters for degradation.Results show as follows:The optimal conditions for the production of reducing sugar in the acid pretreatment process were liquid to material ratios of 10.03,acid concentration of 2.03%,hydrolysis time of 103 min,and the average content of reducing sugar was 19.31%.The proportion of the strains that can greatly increase cellulase activity was Penicillium decumbens and Trichoderma reesei RutC-30,the ratio of inoculation was 3∶7,and the enzymolysis yield was 21.7%.It will provide technical date for the subsequent conversion of the lignocellulose to other products.
作者
邹辉
曾柏全
ZOU Hui;ZENG Bai-quan(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China)
出处
《纤维素科学与技术》
CAS
CSCD
2018年第4期19-27,共9页
Journal of Cellulose Science and Technology
基金
国家林业科学技术推广项目([2016]50)
湖南省科技计划项目(2016NK2149)
国际科学与技术合作项目(2015DFA01120)
关键词
杨木
还原糖
纤维素酶
酶解
poplar
reducing sugar
cellulose
enzymolysis
