摘要
体外纤维重组是天然胶原的重要分子行为,直接影响胶原的性能和应用。本研究以草鱼胶原为研究对象,系统观察了体系温度、pH、离子强度和时间等环境因素对重组胶原纤维形貌和粒径分布的影响。在此基础上,进一步比较了不同环境因子调控下,胶原重组纤维产物的耐酶降解性能和热稳定性能。结果表明,重组胶原纤维粒径和粒径分布受环境因子的调控。中性pH环境下胶原纤维粒径大但分布不均一,体系温度越高、离子强度越大,重组纤维粒径越小,延长纤维重组时间有利于纤维粒径的增大。纤维产物性能分析结果表明,纤维粒径越大,则胶原重组纤维产物的耐酶降解性能和热稳定性能越好。
Fiber reorganization in vitro is an important molecular behavior of natural collagen,which directly affects the performance and application of collagen.In this study,fish collagen extracted from ctenopharyngodon idellus was employed as the research object.The effects of environmental factors such as temperature,pH,ionic strength and incubation time on the morphology and diameter distribution of collagen recombinant fibers were systematically studied.The enzyme degradation resistance and thermal stability of collagen recombinant fiber products under different environmental factors were also compared.It showed that the regulation of environmental factors on the diameter and diameter distribution of collagen recombinant fibers was significant.The collagen fiber has larger diameter but scattered distribution under neutral solution environment.As the temperature rise or the ionic strength increases,the diameter of the recombinant fibers decreases.Prolonging incubation time is beneficial to the increase of fiber diameter.The performance characterization of the fiber reorganization product indicated that the larger fiber diameter,the better enzyme degradation resistance and thermal stability of the collagen recombinant fiber product.
作者
戴蕾
胡秋林
汪海波
徐玉玲
张军涛
未本美
何浪
李盛
许承志
DAI Lei;HU Qiu-lin;WANG Hai-bo;XU Yu-ling;ZHANG Jun-tao;WEI Ben-mei;HE Lang;LI Sheng;XU Cheng-zhi(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;School of Chemical and Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,China)
出处
《武汉轻工大学学报》
2018年第6期14-22,共9页
Journal of Wuhan Polytechnic University
基金
湖北省教育厅科学技术研究计划青年人才项目(Q20181806)
关键词
草鱼
胶原纤维
环境因子
调控
形貌
性能
ctenopharyngodon idellus
collagen fiber
environmental factors
regulation,morphology
performance
