摘要
以黄颡鱼(Pelteobagrus fulvidraco)(14.23±0.65) g为实验对象,分别饲喂3组等氮饲料(C组:组胺含量147 mg/kg; H组:479 mg/kg和H+B组:496 mg/kg+1×10~5 CFU/g罗伊氏乳杆菌Lactobacillus reuteri),进行56d的养殖实验,研究组胺及罗伊氏乳杆菌对黄颡鱼的影响。结果显示, H+B组血清谷丙转氨酶(ALT)及谷草转氨酶(AST)活力显著低于H组(P<0.05),而与C组无差异(P>0.05); H+B组肝脏谷胱甘肽S转移酶(GSH-ST)活力及丙二醛(MDA)含量显著低于H组(P<0.05),与C组无差异(P>0.05);肝脏HE染色显示, H组肝脏中有较多炎症细胞浸润,而H+B组肝脏细胞结构完整,无明显损伤;肠道HE染色显示, H组肠道皱襞数量明显减少、长度显著变短,有水肿发生,而H+B组肠道皱襞数量、长度与对照组无差异,水肿程度不明显。结果表明:组胺会引起黄颡鱼肝脏、肠道损伤,而罗伊氏乳杆菌对组胺引发的肝肠损伤有保护功效。
To investigate the effects of histamine and Lactobacillus reuteri on Pelteobagrus fulvidraco,three isonitrogenous diets,namely diet C(control,with a 147 mg/kg content of histamine),diet H(with a 479 mg/kg content of histamine)and diet H+B(histamine+bacteria:a 496 mg/kg content of histamine and 1×105 CFU/g L.reuteri),were formulated for a 56-day trial of P.fulvidraco juveniles(14.23±0.65)g.Our data indicated that plasma alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity were significantly lower in group H+B compared with those of group H(P<0.05),while there was no significant difference between group H+B and group C(P>0.05).Meanwhile,liver glutathione-s-transferase(GSH-ST)activity and malondialdehyde(MDA)content were dramatically reduced in group H+B compared with those of group H(P<0.05),while there was no significant difference between group H+B and group C(P>0.05).Furthermore,the HE staining showed that there were inflammatory cells infiltrating in the liver of fish fed with diet H,while no hepatocyte abnormality observed in group C and group H+B,and that the intestinal folds decreased significantly with shorter and edema in diet H group,while this is no marked difference in the length of intestinal folds between group C and H+B.Overall,our studies indicated the protective effect of L.reuteri against histamine in cultured fish diet.
作者
成艳波
李薇
韩木兰
许国焕
谢黎炜
印遇龙
梁建庆
CHENG Yan-Bo;LI Wei;HAN Mu-Lan;XU Guo-Huan;XIE Li-Wei;YIN Yu-Long;LIANG Jian-Qing(State Key Laboratory of Applied Microbiology,South China&Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China;Bide Biotechnology Co.,Ltd.of Guangdong,Guangzhou 510663,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2019年第1期94-101,共8页
Acta Hydrobiologica Sinica
基金
2017年广东科技发展专项(协同创新及平台环境建设)(2017B090901052)
广东省科学院引进高层次领军人才专项资金项目(2016GDASRC-0202)资助~~
关键词
黄颡鱼
罗伊氏乳杆菌
组胺
HE染色
保护功效
Pelteobagrus fulvidraco
Lactobacillus reuteri
Histamine
HE stain
Protective efficacy
