摘要
在实验室条件下,首次发现了带形蜈蚣藻(Grateloupia turuturu)盘状体产生的丝状体能够形成孢子,暂命名为"盘丝体孢子"。研究详细观察了该盘丝体孢子的形成过程,并探讨了不同温度6、12、16、20、24和30℃及不同光照强度10、30、45、60、90和120μmol/(m^2·s)对盘丝体孢子放散的影响。结果表明:(1)带形蜈蚣藻雌配子体的囊果释放果孢子,果孢子发育形成盘状体,盘状体经过诱导再生出单列细胞的丝状体,丝状体形成多室孢子囊,并放散出大量盘丝体孢子;(2)温度和光照强度均对丝状体中盘丝体孢子的放散产生显著影响。在温度为16℃、光照强度为60μmol/(m^2·s)时盘丝体孢子放散量有最大值;(3)在温度低于12℃或高于24℃时,盘丝体孢子的放散受到影响,数量明显减少;(4)在光照强度低于30μmol/(m^2·s)或高于90μmol/(m^2·s)时,盘丝体孢子的放散明显受到抑制。研究结果补充了带形蜈蚣藻无性繁殖过程,为其种质保存、人工育苗及养殖提供更为丰富的理论依据,为探讨带形蜈蚣藻的起源与演化提供新思路。
We discovered for the first time that Grateloupia turuturu filaments,developed from discoid crusts,had the ability to form spores under laboratory condition,which defined as“disc-filamentospore”.We investigated the formation of disc-filamentospores and the effects of various temperatures(6,12,16,20,24,30℃)and light intensities(10,30,45,60,90,120μmol/(m2.s))on the release of disc-filamentospores.The results indicated that carpospores that formed and released from cystocarps of female gametophyte of G.turuturu,first developed into discoid crusts.The discoid crusts were induced to form filaments of monolayer cells,which formed massive plurilocular sporangiums and released large amounts of disc-filamentospores.Temperature and light intensity significantly affected the releasing efficiency of disc-filamentospores with the maximum level at 16℃and 60μmol/(m2·s).The releasing number of disc-filamentospores reduced when the temperature was lower than 12℃or higher than 24℃.The light intensity with less than 30μmol/(m2·s)or more than 90μmol/(m2·s)significantly inhibited the releasing efficiency of disc-filamentospores.Our results improved the asexual reproduction process of Grateloupia turuturu and provided more abundant theoretical basis for its germplasm preservation,artificial breeding and aquaculture.Finally,we suggest a plausible solution to investigate the origin and evolution of Grateloupia turuturu.
作者
陈蕾
卞瑶
周旭
娄宇
依朋
王宏伟
CHEN Lei;BIAN Yao;ZHOU Xu;LOU Yu;YI Peng;WANG Hong-Wei(College of Life Sciences,Liaoning Normal University,Dalian 116081,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2019年第1期219-225,共7页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(31270251和31570209)资助~~
关键词
带形蜈蚣藻
丝状体
盘丝体孢子
温度
光照强度
孢子放散
Grateloupia turuturu
Filament
Disc-filamentospore
Temperature
Light intensity
Releasing of spores
