摘要
目的观察氢吗啡酮后处理对缺血-再灌注大鼠体外心肌再灌注性心律失常及心肌组织缝隙连接蛋白43(Cx43)表达的影响,并探讨其相关机制。方法 SD大鼠24只,随机分为3组:对照组(C组)、缺血-再灌注组(IR组)和氢吗啡酮后处理组(HM组),每组8只。建立Langendorff体外心脏缺血-再灌注模型,连续监测心电图和心率。分析再灌注时心律失常发生情况并评分,采用Western blotting技术检测缺血-再灌注心室肌Cx43和Akt信号通路蛋白的表达。结果 3组大鼠各时点心率组间、组内比较均差异无统计学意义(P>0.05)。再灌注期间,IR组和HM组的室性期前收缩数量和再灌注心律失常评分差异无统计学意义(P>0.05);与IR组比较,HM组发生室速和室颤的大鼠数更少,再灌注时心律失常的持续时间更短(P<0.05)。与C组比较,IR组Akt的表达差异无统计学意义(P>0.05),HM组的Akt表达上调,IR组和HM组心肌Cx43的表达均下调;与IR组比较,HM组心肌Akt和Cx43的表达均上调(P<0.05)。结论氢吗啡酮后处理抑制大鼠心肌缺血-再灌注损伤诱发的心律失常机制与其激活Akt信号通路后上调心肌Cx43表达有关。
Objective To investigate the effect of hydromorphone postconditioning on the reperfusion arrhythmia and the expression of myocardial connexin43(Cx43)during ischemia-reperfusion in isolated rat hearts and its mechanism.Methods The SD rats(n=24)were randomly divided into three groups:control group(group C,n=18),ischemia-reperfusion group(group I/R,n=8),hydromorphone post-treatment group(group HM,n=8).The langendorff heart or isolated perfused heart assay was established.The heart rate(HR)and ECG during the whole experiment period were recorded.And the reperfusion arrhythmia during the period of reperfusion were detected by ECG.The expression of Akt and Cx43protein were detected by Western blotting technique.Results Although the HR at different time points and the reperfusion arrhythmia score of three groups were not statistically significant(P>0.05),the duration of reperfusion arrhythmia of group HM was significantly shorter than that of group IR,and the incidence of ventricular tachycardia and ventricular fibrillation in group HM were also less than that in group IR(P<0.05).When Akt expression level in group IR was compared with group C,there was no significant differences(P>0.05),but Akt expression level in group HM was obviously increased;Cx43expression level in group IR and group HM were both significantly reduced.While compared with the group IR,Cx43expression level in group HM was significantly increased(P<0.05).Conclusion The mechanism by which hydromorphone post-treatment inhibits reperfusion arrhythmia induced by myocardial IR is associated with up-regulated expression of myocardial Cx43after activation of Akt signaling pathway during ischemia-reperfusion in isolated rat hearts.
作者
易菁
段宏伟
高鸿
曾庆繁
王子君
王贵龙
刘艳秋
YI Jing;DUAN Hongwei;GAO Hong;ZENG Qingfan;WANG Zijun;WANG Guilong;LIU Yanqiu(Department of Anesthesiology, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004,China;Department of Anesthesiology, the Affiliated Pudong Hospital of Fudan University, Shanghai 201301,China;School of Anesthesiology, Guizhou Medical University, Guiyang 550004, China)
出处
《医药导报》
CAS
北大核心
2019年第1期9-13,共5页
Herald of Medicine
基金
贵阳市科技计划项目(筑科合同[20151001]社31号)
贵州省科技厅联合基金资助项目(黔科合LH字[2015]7424号)
贵州省麻醉学专业学位研究生工作站(黔教研合JYSZ字[2015]006)
