摘要
目的探讨针刺肾俞足三里防治绝经后骨质疏松症(PMOP)的可能作用机制。方法 50只雌性SD大鼠随机分为空白组、假手术组、模型组、肾俞足三里组和非经非穴组。行双侧卵巢切除术复制骨质疏松模型。肾俞足三里组、非经非穴组每天针刺1次,留针30 min,10次1疗程,疗程间期5天,6个疗程后同时处死5组大鼠,取右侧胫骨测量胫骨、胫骨上端骨密度;RTPCR法检测大鼠腰椎c-myc、Cyclin D1和R unx2 mR NA表达。结果与假手术组比较,模型组胫骨、胫骨上端骨密度降低(P<0.05);与模型组比较,肾俞足三里组胫骨、胫骨上端骨密度升高(P<0.05),非经非穴组胫骨、胫骨上端骨密度差异均无统计学意义(P>0.05);c-myc mRNA表达:肾俞足三里组c-myc mRNA表达与假手术组比较差异无统计学意义(P>0.05);各实验组Cyclin D1灰度值均值差异均无统计学意义(P>0.05);Runx2 mRNA表达:与空白组比较,模型组Runx2 mRNA表达降低(P<0.05),与模型组比较,肾俞足三里组与非经非穴组的表达升高(P<0.05)。结论针刺肾俞、足三里穴可改善绝经后骨质疏松的骨密度降低,其作用机制可能与调节腰椎c-myc、Cyclin D1和Runx2 mRNA表达有关。
Objective To explore the possible mechanisms of acupuncture at Shenshu(BL23)and Zusanli(ST36)for the treatment of postmenopausal osteoporosis(PMOP).Methods Fifty female SD rats were randomly divided into a blank group,a shamoperation group,a model group,a acupoint(BL 23 and ST36)group and a non-acupoint group.The osteoporotic rat model was established by bilateral ovariectomy.The acupoint group and non-acupoint group were given acupuncture once per day.10 treatments made one session.There were 5 days at the interval between two sessions.All rats were killed and the bone mineral density(BMD)of the right tibias and proximal tibias were obtained after 6 sessions.Meanwhile,the expression of cmyc,Cyclin D1,and Runx2 mRNA in lumbar vertebra were detected by RT-PCR.Results BMD of the right tibias and proximal tibias in the model group were lower than those in the sham-operation group(P<0.05).Compared with the model group,the BMD of the right tibias and proximal tibias in the acupoint group were obviously increased(P<0.05),but no significant difference in the non-acupoint group(P>0.05).Compared with the sham-operation group,the expression of cmyc mRNA of the acupoint group had no significant differences(P>0.05).The difference in the mean grey values of Cyclin D1 in each experimental group was not statistically significant(P>0.05).The expression of Runx2 mRNA in the model group were lower than those in the blank group(P<0.05).Compared with the model group,the expression of Runx2 mRNA in the acupoint group and non-acupoint group were obviously increased(P<0.05).Conclusion Acupuncture at Shenshu(BL23)and Zusanli(ST36)could reduce PMOP and increase BMD.This action is probably associated with the regulation of the expression of cmyc,Cyclin D1,and Runx2 mRNA in lumbar vertebra.
作者
李俐
郑旭仙
曾雅芬
余晓雯
吴明霞
LI Li;ZHENG Xuxian;ZENG Yafen;YU Xiaowen;WU Mingxia(Department of Acupuncture,the Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350003,China;College of Acupuncture-Moxibustion,Fujian University of Traditional Chinese Medicine,Fujian Province,Fuzhou 350122,China)
出处
《中国中医药现代远程教育》
2019年第1期107-111,共5页
Chinese Medicine Modern Distance Education of China
基金
国家自然科学基金面上项目【No.81373719】
福建省自然科学基金【No.2015J01479】
关键词
肾俞
足三里
绝经
骨质疏松症
骨密度
动物实验
痹证
Shenshu(BL23)
Zusanli(ST36)
postmenopausal
osteoporosis
bone mineral density
animal experiment
arthromyodynia
