胃癌病灶内PCDH10对癌细胞浸润性生长及上皮间质转化的影响被引量：2

Effect of PCDH10 on infiltrative growth and epithelial-mesenchymal transition of cancer cells in gastric cancer

下载PDF

导出

摘要目的:研究胃癌病灶内编码原钙黏蛋白10(PCDH10)对癌细胞浸润性生长及上皮间质转化的影响。方法:选择在攀枝花市中心医院接受手术切除的胃癌患者作为研究对象,手术切除后留取胃癌病灶及癌旁病灶,测定PCDH10基因及上皮间质转化基因的mRNA表达量、蛋白酶及其抑制分子的蛋白含量。结果:胃癌病灶内PCDH10、TIMP1、TIMP2、RASAL2、E-cadherin的mRNA表达量显著低于癌旁病灶,Vav2、Vav3、CatB、MMP9、ZEB1、Twist1、Vimentin的含量显著高于癌旁病灶;PCDH10低表达的胃癌病灶内Vav2、Vav3、CatB、MMP9、ZEB1、Twist1、Vimentin的含量显著高于PCDH10高表达的胃癌病灶,TIMP1、TIMP2、RASAL2、E-cadherin的含量显著低于PCDH10高表达的胃癌病灶。结论:胃癌病灶内PCDH10低表达对癌细胞浸润性生长及上皮间质转化具有促进作用。 Objective: To study the effect of protocadherin 10 (PCDH10) on infiltrative growth and epithelial-mesenchymal transition of cancer cells in gastric cancer. Methods: The patients with gastric cancer who underwent surgical resection in Panzhihua Central Hospital between July 2014 and February 2018 were selected as the research subjects, and the gastric cancer lesions and adjacent lesions were collected after surgical resection to determine the mRNA expression of PCDH10 gene and epithelial-mesenchymal transition genes as well as the protein levels of proteases and their inhibitory molecules. Results: PCDH10, TIMP1, TIMP2, RASAL2 and E-cadherin mRNA expression levels in gastric cancer lesions were significantly lower than those in adjacent lesions whereas Vav2, Vav3, CatB, MMP9, ZEB1, Twist1 and Vimentin contents were significantly higher than those in adjacent lesions; Vav2, Vav3, CatB, MMP9, ZEB1, Twist1 and Vimentin contents in gastric caner lesions with lower PCDH10 expression were significantly higher than those in gastric caner lesions with higher PCDH10 expression whereas TIMP1, TIMP2, RASAL2 and E-cadherin contents were significantly lower than those in gastric caner lesions with higher PCDH10 expression. Conclusion: The low expression of PCDH10 in gastric cancer can promote the infiltrative growth and epithelial-mesenchymal transition of cancer cells.

作者刘培根 LIU Pei-gen(Department of General Surgery, Panzhihua Central Hospital in Sichuan Province, Panzhihua, Sichuan Province, 617000, China)

机构地区四川省攀枝花市中心医院普外科

出处《海南医学院学报》 CAS 2018年第24期2137-2139,2144,共4页 Journal of Hainan Medical University

基金 2015年度四川省科技厅项目(2015080514)~~

关键词胃癌原钙黏蛋白10 蛋白酶上皮间质转化 Gastric cancer Protocadherin 10 Protease Epithelial-mesenchymal transition

分类号 R735.2 [医药卫生—肿瘤]

引文网络
相关文献

参考文献2

1何琳莉,蹇顺海,文彬.cathepsin B与cystatin C在青年及老年人胃癌发生、发展中的意义[J].临床与实验病理学杂志,2016,32(4):380-383. 被引量：6
2赵猛,赵晓朋,李峥,任丽.RASAL2对胃癌细胞侵袭与上皮间质转化的影响[J].中国肿瘤临床,2016,43(16):691-696. 被引量：6

二级参考文献17

1Tan G J, Peng Z K, Lu J P, Tang F Q. Cathepsins mediate tumor metastasis[J]. World J Biol Chem, 2013,4(4) :91 -101.
2Aggarwal N, Sloane B F. Cathepsin B: multiple roles in cancer [J]. Proteomics Clin Appl, 2014,8(5-6) :427 -37.
3Bian B, Mongrain S, Cagnol S, et al. Cathepsin B promotes col.or- ectal tumorigenesis, cell invasion, and metastasis [ J/OL]. Mol Carcinog, 2015. doi: 10. 1002/mc. 22312. [ Epub ahead of print ].
4Swisher L Z, Prior A M, Gunaratna M J, et al. Quantitative elec- trochemical detection of cathepsin B activity in breast cancer cell lysates using carbon nanofiber nanoelectrode arrays toward identifi- cation of cancer formation [ J ]. Nanomedicine, 2015,11 (7) : 1695 - 704.
5Alapati K, Kesanakurti D, Rao J S, Dasari V R. uPAR and ca- thepsin B-mediated compartmentalization of JNK regulates the mi- gration of glioma-initiating cells [ J 1. Stem Cell Res, 2014, 12 (3) :716 -29.
6Ma W, Ma L, Zhe H, et al. Detection of esophageal squamous cell carcinonm by cathepsin B activity in nude mice [ J 1. PLoS One, 2014,9(3) :e92351.
7Gong F, Peng X, Luo C, et al. Cathepsin B as a potential prog- nostic and therapeutic marker for human lung squamous cell carci- noma[Jl. Mol Cancer, 2013,12(20) :125.
8Wu D, Wang H, Li Z, et al. Cathepsin B may be a potential bio- marker in cervical cancer [ J ]. Histol Histopathol, 2012,27 ( 1 ) : 79 - 87.
9Wegiel B, .Iiborn T, Abrahamson M, et al. Cystatin C is down- regulated in prostate cancer and modulates invasion of prostate cancer cells via MAPK/Erk and androgen receptor pathways [ J ]. PLoS One, 2009.4 ( 11 ) : e7953.
10Kim J T, Lee S J, Kang M A, et al. Cystatin SN neutralizes the inhibitory effect of cystatin C on cathepsin B activity [J]. Cell Death Dis, 2013,4(6) :e974.