摘要
【目的】研究口含烟对人口腔卫生的影响。【方法】将3种不同类型口含烟(胶基型、袋装型、含片型)提取物和传统卷烟烟气总粒相物(TPM)基于烟碱含量配成不同浓度,研究其对变形链球菌和牙龈卟啉单胞菌的抑菌效应。选取20名志愿者吸食3种口含烟和传统卷烟,以吐取法采集吸食前5 min和吸食后0～2min,2～5 min,5～10 min,10～15min,90～95 min共6个时间段的唾液,提取唾液中细菌DNA,通过实时荧光定量PCR(探针法)检测各时间节点总细菌、变形链球菌和牙龈卟啉单胞菌水平。【结果】(1)3种口含烟在体外对2种细菌无抑菌作用,普通卷烟TPM有一定抑菌作用。(2)吸食袋装型、含片型口含烟和传统卷烟后,唾液中细菌总量短期有所降低,但1 h后均恢复至使用前水平。牙龈卟啉单胞菌与变形链球菌量变化趋势与总细菌基本一致。【结论】口含烟及传统卷烟在吸食后短期内对口腔微生物有抑制作用。
To explore the effect of oral smokeless tobacco(SLT)product on oral hygiene.Extracts of three different types of SLT,i.e.gel-based-type,bag-type,and flake-type,and traditional cigarette smoke total particulate matter(TPM)were formulated into different concentrations based on nicotine content,and antibacterial effect on Streptococcus mutans and Porphyromonas gingivalis was studied.Twenty volunteers were asked to take 3 types SLT or traditional cigarettes.Results were collected 5 minutes before intake and 0~2min,2~5min,5~10min,10~15min,90~95 min.after intake of tobacco.Bacterial DNA in saliva was extracted,and total bacteria,Streptococcus mutans and Porphyromonas gingivalis levels at each time point were determined by real-time fluorescent quantitative PCR(probe method).Results showed:(1)3 types SLT had no significant effect on the numbers of Porphyromonas gingivali and Streptococcus mutans,but TPM had some inhibitory effect.(2)Total bacteria in saliva slightly decreased in the short-term after using SLT(gel-based-type and flake-type)and traditional cigarette,but after 1h both returned to pre-use levels.The change of Porphyromonas gingivalis and Streptococcus mutans was almost consistent with that of total bacteria.It was concluded that the use of SLT and traditional cigarettes will have a short-term inhibitory effect on oral microbiological content.
作者
高茜
管莹
米其利
黄海涛
杨继
徐玉琼
简小朋
夭建华
GAO Qian;GUAN Ying;MI Qili;HUANG Haitao;YANG Ji;XU Yuqiong;JIAN Xiaopeng;YAO Jianhua(China Tobacco Yunnan Industrial Co. Ltd.,Kunming 650106,China;Shanghai New Tobacco Product Research Institute Co.,Ltd.,Shanghai 200082,China)
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2018年第6期110-115,共6页
Acta Tabacaria Sinica
基金
云南中烟工业有限责任公司项目(2018XY04以舒适感和口腔健康为导向的无烟气烟草关键技术研究)
中国烟草总公司项目(110201801011(XX-11)基于袋装口含烟的天然植物与烟草配伍机理及加工工艺研究
