摘要
采用高纯度CO_2和空气的混合气体调配试验所需酸化海水,研究不同海水酸化条件胁迫对脊尾白虾超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)浓度的影响.结果表明,1 000μL/L CO_2酸化组SOD,CAT和GSH-Px活性表现为先上升后下降的趋势,其中SOD和CAT活性分别在7d和14d时受到显著诱导(P<0.05),而GSH-Px活性在7d和14d时显著高于对照组(P<0.05);1 900μL/L酸化组SOD,CAT和GSHPx活性在暴露过程中受到不同程度的抑制,其中SOD活性在14d和28d时均显著低于对照组(P<0.05),CAT和GSH-Px活性均在14d时受到显著抑制(P<0.05);1 000μL/L和1 900μL/L CO_2酸化组的MDA浓度均呈现先增加后回落的趋势,1 000μL/L组在14d时MDA浓度显著增加(P<0.05),而1 900μL/L组则在7d和14d时MDA浓度均显著高于对照组(P<0.05)且在14d时达到最大值.由此可见,海洋酸化对脊尾白虾的抗氧化酶活性具有明显的刺激作用,且在轻度酸化胁迫时抗氧化酶活性受到诱导,而在较高强度的酸化胁迫时其活性被抑制.
The effects of different seawater acidification conditions on superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)concentration of Exopalaemon carinicauda were studied by mixing high purity CO2 with air.The results showed that activities of SOD,CAT,and GSH-Px in 1 000μL/L CO2 acidification treatment were increased at first and then decreased,and the activities of SOD and CAT were significantly induced at 7 and 14 d(P<0.05),respectively.The activity of GSH-Px in 1 000μL/L group was significantly higher than that in control group at 7 and 14 d(P<0.05).However,the activities of SOD,CAT,and GSH-Px in 1 900μL/L CO2 acidification group were inhibited.The activity of SOD was significantly lower in 1 900μL/L CO2 acidification group than that in control group at 14 and 28 d(P<0.05),and the activity of CAT and GSH-Px were remarkably restrained at 14 d(P<0.05).The MDA content were increased at first and then decreased in 1 000μL/L and 1 900μL/L CO2 acidification groups.MDA content in 1 000μL/L group was evidently increased at 14 d(P<0.05),and it was observably higher in 1 900μL/L group than that of control group at 7 and 14 d(P<0.05).These results indicate that seawater acidification can evidently stimulate the activities of antioxidant enzyme in E.carinicauda,and these enzyme activities are induced in mild acidification stress but they are inhibited when acidification is enhanced.
作者
李晨辉
王梦杰
彭艳青
高焕
王怀忠
王海华
陈建华
LI Chenhui;WANG Mengjie;PENG Yanqing;GAO Huan;WANG Huaizhong;WANG Haihua;CHEN Jianhua(School of Marine Life and Fisheries,Huaihai Institute of Technology,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China;Lianyungang Zhongyu Aquaculture Co.,Ltd.,Lianyungang 222100,China;Jiangxi Institute of Aquatic Science,Nanchang 330039,China)
出处
《淮海工学院学报（自然科学版）》
CAS
2018年第4期78-83,共6页
Journal of Huaihai Institute of Technology:Natural Sciences Edition
基金
江苏高校品牌专业建设工程一期项目(PPZY2015B159)
广东省渔业生态环境重点实验室开放基金项目(LFE-2015-10)
江苏省"六大人才高峰"创新人才团队资助项目(2016-HYGC-CXTD-004)
