摘要
以白及多糖粗提液为对象,考察絮凝剂ZTC-Ⅱ絮凝纯化工艺。在单因素试验的基础上,运用PlackettBurman(PB)试验,选择出具有显著影响的因素。通过满意度函数与响应面相结合的分析方法,建立各影响因素的二次回归方程模型,得到白及多糖的最佳纯化条件:絮凝温度为48℃,絮凝剂ZTC-Ⅱ组分B用量为0. 77 m L/g,组分A、B用量比为0. 34∶1,絮凝时间为1. 5 h,浓缩比为1∶12 g/m L。在此优化工艺下,白及多糖保留率为(92. 32±0. 34)%,脱蛋白率为(51. 43±0. 12)%。
Flocculating purification for crude polysaecharide extracting solution of Bleailla striata by ZTC-Ⅱflocculant was studied.The main influence factors were selected by Plackett-Burman(PB)test on the basis of these single factors.From the results of response surface methodology combined with desirability function,the regression equation of each influencing factor was established.The optimum purification conditions of crude Bleailla striata polysaccharide were as follows:flocculation temperature of 48℃,dosage of component B of 0.77 mL/g,ratio of component A and B of 0.34∶1,flocculation time of 1.5 h,and ratio of mass to volume of 1∶12 g/mL.Under such optimized conditions,the retention rate of polysaccharide was(92.32±0.34)%,and deproteinizing rate was(51.43±0.12)%.
作者
彭茹洁
赵少甫
王承潇
韩伟
PENG Rujie;ZHAO Shaofu;WANG Chengxiao;HAN Wei(Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;School of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2019年第1期57-63,共7页
Journal of Nanjing Tech University(Natural Science Edition)
基金
云南省科技重大专项(2018ZF011-2018110506)
关键词
白及多糖
絮凝纯化
满意度函数
响应面
Bleailla striata polysaecharide
flocculating purification
desirability function
response surface methodology
