摘要
[目的]研究细叶石仙桃提取物石油醚部位对自由基的清除活性。[方法]采用石油醚溶剂得到细叶石仙桃石油醚提取物,并研究其对DPPH自由基和羟自由基的清除能力。以芦丁为对照品,测定其总黄酮含量。[结果]细叶石仙桃石油醚提取物对DPPH清除率在高药液浓度时达到最大值,对羟自由基清除率最高可达到100%。[结论]细叶石仙桃石油醚提取物具有优异的自由基清除活性。
[Objective]The aim was to study the radical clearance ability of petroleum ether extract of Pholidota cantonensis Rolfe.[Method]The petroleum ether extract of Pholidota cantonensis Rolfe were obtained using petroleum ether.The radical clearance abilities of petroleum ether extract of Pholidota cantonensis Rolfe were studied by DPPH assay and hydroxyl radical assay.The total flavone content was determined using rutin as reference substance.[Result]The extract of Pholidota cantonensis Rolfe showed the highest clearance rate against DPPH radicals at higer concentration of extract,and showed a 100%clearance rate against hydroxyl radicals.[Conclusion]The petroleum ether extract of Pholidota cantonensis Rolfe possesses excellent radical clearance ability.
作者
李培源
莫媛媛
彭炳华
霍丽妮
秦一兰
LI Pei-yuan;MO Yuan-yuan;PENG Bing-hua(College of Pharmacy, Guangxi University of Chinese Medicaine, Nanning,Guangxi 530001;The 56th Middle School, Nanning,Guangxi 530001;College of Chemistry and Materials Science, Guangxi Teachers Education University, Nanning ,Guangxi 530001)
出处
《安徽农业科学》
CAS
2019年第1期174-175,181,共3页
Journal of Anhui Agricultural Sciences
基金
广西自然科学基金(2017GXNSFAA198335)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西壮瑶药重点实验室(桂科基字[2014]32号)
广西重点学科壮药学(桂教科研[2013]16号)
广西八桂学者中药创新理论与药效研究(J13162)
广西中医药大学校级课题(2017JQ001)
广西中医药大学中药学优势学科建设专项课题(ZYX2017003)
杨世林教授团队人才培养建设项目(YSL17004)
关键词
细叶石仙桃
DPPH
羟自由基
清除能力
总黄酮含量
Pholidota cantonensis Rolfe
DPPH
Hydroxyl radical
Clearance ability
Total flavone
