摘要
采用超声辅助法提取黑菊芋中多酚化合物,选择乙醇体积分数、提取温度、提取时间及料液比4个因素进行单因素实验,并采用正交试验优化提取工艺,确定了黑菊芋总多酚超声提取的较优条件为乙醇体积分数55%、料液比(g/mL) 1:35、温度85℃、时间45 min,此条件下的提取率为6. 26 mg/g。利用此工艺条件提取菊芋多酚,检测结果以干基计算,鲜菊芋的菊芋多酚质量比为1. 13 mg/g,黑菊芋的为6. 41 mg/g,是鲜菊芋的5. 67倍。体外抗氧化实验结果表明,黑菊芋各抗氧化指标均与多酚含量具有较好的相关性,其中还原力相关性最高为0. 994 6。不同作用量时,黑菊芋对DPPH.自由基、羟自由基的清除率和铁离子还原力均显著高于鲜菊芋,分别为同质量鲜菊芋(干重计)的1.08～1.54倍,1.02～1.28倍和2. 56～3. 98倍。
The orthogonal experiment was used to determine the optimization extraction condition of polyphenols from black and fresh Jerusalem artichoke.The optimal conditions were ethanol concentration 55%,the material solvent ratio 1∶35 g/mL,temperature 85℃,and time 45 min.Under this condition,the polyphenols extraction yield of black Jerusalem artichoke was 6.41 mg/g while the extraction yield of fresh Jerusalem artichoke was 1.13 mg/g.The results also showed that the antioxidant activities had relevance with polyphenols contents and a positive correlation between polyphenols contents and ferric reducing antioxidant power was obtained(R=0.994 6).Polyphenols from black Jerusalem artichoke had higher DPPH scavenging ability,hydroxyl radical scavenging ability,and ferric reducing antioxidant power,which were 1.08~1.54 times,1.02~1.28 times,and 2.56~3.98 times higher than fresh Jerusalem artichoke.
作者
姬妍茹
许博超
杨庆丽
魏连会
石杰
董艳
刘宇峰
JI Yanru;XU Bochao;YANG Qingli;WEI Lianhui;SHI Jie;DONG Yan;LIU Yufeng(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,China;College of Life Science and Technology,Heilongjiang Bayi Agriculture University,Daqing 163319,China)
出处
《食品科学技术学报》
CAS
CSCD
北大核心
2019年第1期84-89,共6页
Journal of Food Science and Technology
基金
黑龙江省院所基本应用技术研究专项(ZNB22017DQ01)
关键词
菊芋
黑菊芋
多酚
超声辅助提取
体外抗氧化
Jerusalem artichoke
black Jerusalem artichoke
polyphenols
ultrasonic wave-assisted extraction
in vitro antioxidation
