摘要
【目的】采用GB5009. 9-2016中酶水解法测定滇黄精淀粉含量的研究。【方法】通过单因素试验、正交试验对脱糖次数、酶解时间、酶解温度和用酶量的研究,优化了滇黄精淀粉提取测定工艺。【结果】酶水解法提取测定滇黄精淀粉含量的最佳工艺条件为脱糖5次、淀粉酶量15 m L(5 g/L)、酶解温度57℃、酸水解55 min。【结论】优化方案是测定滇黄精淀粉含量的最佳工艺,误差最小。为进一步开展滇黄精淀粉研究提供了基础数据。
【Objective】The standard GB5009.9-2016 for enzymatic hydrolysis was used to analyze the starch of Polygonatum kingianum.【Method】In order to obtain the optimum removal technologies of the determination of starch from P.kingianum,the single factor and orthogonal experiments of the desugar times,enzyme concentration,enzymolysis time and enzymolysis temperature were analyzed.【Result】The result showed that the optimization technology was the desugar times 5 times,enzyme concentration 15 mL(5g/L),enzymolysis temperature 57℃and acid hydrolysis 55 min.【Conclusion】It would set a basic date to develop the study of the starch of P.kingianum.
作者
李彦莹
朱新焰
石亚娜
杨发建
季鹏章
王家金
LI Yan-ying;ZHU Xin-yan;SHI Ya-na;YANG Fa-jian;JI Peng-zhang;WANG Jia-jin(College of Food Sciences and Technology,Yunnan Agricultural University,Yunnan Kunming 650223,China;Institute of Medical Plant,Yunnan Academy of Agricultural Science,Yunnan Kunming 650092,China;Baoshan College of Traditional Chinese Medicine,Yunnan Baoshan 678000,China)
出处
《西南农业学报》
CSCD
北大核心
2018年第12期2533-2536,共4页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31760347)
云南省科技计划项目(2015FB161)
云南省级环境保护专项资金项目(2014BI006)
昆明市生物产业项目(2016-17)
两类人才(2018HB085)
关键词
酶水解法
滇黄精
淀粉含量
淀粉酶
优化
Enzymatic hydrolysis
Polygonatum kingianum
Content of starch
Amylase
Optimization
