摘要
目的评价知黄溃疡合剂对小鼠的急性毒性作用,为临床安全用药提供理论依据。方法小鼠禁食不禁水16h,以最大体积(每克体质量0.04mL计),最大质量浓度为1.5g·mL-1,24h内灌胃给药知黄溃疡合剂2次,观察并记录给药后14d内小鼠的毒性反应情况;实验末期检测小鼠的生化指标,处死小鼠,解剖并进行观察,取心、肝、脾、肺和肾,称定湿质量并计算脏器指数。结果小鼠灌胃知黄溃疡合剂120g·kg-1原生药后无死亡,生化指标与对照组比较差异无统计学意义,脏器指数与对照组比较差异无统计学意义。结论小鼠灌胃知黄溃疡合剂无明显急性毒性,最大耐受量为120g·kg-1原生药,相当于临床拟用剂量的109.1倍。
Objective To evaluate the acute toxicity of Zhihuang Ulcer Mixture in mice,and to provide a scientific basis for its clinical application.Methods Mice,fasting for solids but not water for 16 h,were given Zhihuang Ulcer Mixture 2 times at intervals in 24 h,with the maximum volume(0.04 mL per gram body weight)and the maximum concentration of 1.5 g·mL^-1,then the toxicity of the mice within 14 d were observed and recorded.The biochemical indexes,the wet weight of heart,liver,spleen,lung and kidney,and the viscera index at the end of experiment were determined.Results The mice didn′t die after given the crude drug of Zhihuang Ulcer Mixture at the amount of 120 g·kg^-1,there was no statistical difference in the biochemical indicators or the viscera index when compared with control group.Conclusion There is no obvious acute toxicity observed in mice which was given the crude drug of Zhihuang Ulcer Mixture within the maximum volume of 120 g·kg^-1,equivalent to 109.1 times in the clinical application for human.
作者
胡晓燕
唐志立
郭廷东
HU Xiaoyan;TANG Zhili;GUO Tingdong(Department of Pharmacy,Nanchong Central Hospital;Department of Pharmacy,the Second Clinical Hospital of North Sichuan Medical University,Nanchong 637000,China)
出处
《西北药学杂志》
CAS
2019年第1期65-67,共3页
Northwest Pharmaceutical Journal
基金
2014年度四川省科技厅资助项目(编号:2014SZ0140)
