摘要
本实验分别采用六氟异丙醇(HFIP)、HFIP-甲酸(体积比8∶2)以及HFIP-二氯甲烷(体积比8∶2)作为聚己内酯(PCL)的溶剂,采用HFIP作为丝素(SF)的溶剂,利用同轴静电纺丝技术制备皮/芯结构的SF/PCL(C-SF/PCL)纤维。透射电子显微镜的测试结果显示,所制备的纤维网中具有C-SF/PCL纤维。X射线能谱仪的分析结果表明,当芯层PCL采用HFIP-二氯甲烷(体积比8∶2)作为溶剂时,纤维网中含有较多的C-SF/PCL纤维。拉伸测试结果表明,C-SF/PCL纤维网的拉伸断裂强度和断裂伸长率都较纯SF纤维网有明显提高。以纤维网作为支架培养EA.hy926细胞,结果表明,C-SF/PCL纤维网和SF纤维网相似,细胞都能良好黏附和增殖。以上研究结果说明,C-SF/PCL纤维网可能有潜力应用于血管组织工程支架。
In this work,hexafluoroisopropanol(HFIP)-formic acid(volume ratio 8∶2),HFIP,and HFIP-dichloromethane(volume ratio 8∶2)were used to dissolve PCL as the core solutions,and HFIP was used to dissolve SF as the sheath solution.Then,skin-core structured SF/PCL(C-SF/PCL)fibers were prepared by coaxial electrospinning with the core and sheath solutions.TEM images indicated the existence of the core-shell structure of the fibers,and EDAX results revealed that the fiber mat with the greatest content of core-shell structure fibers was obtained when the core solvent was HFIP-dichloromethane(volume ratio 8∶2).Tensile tests showed that the C-SF/PCL fiber mat displayed improved tensile properties,with strength and elongation that were significantly higher than those of the pure SF mat.The C-SF/PCL fiber mat was further investigated as a scaffold for culturing EA.hy926 cells,and the results showed that the fiber mat permitted cellular adhesion,proliferation and spreading in a manner similar to that of the pure SF fiber mat.These results indicated that the coaxial electrospun SF/PCL fiber mat could be considered a promi-sing candidate for tissue engineering scaffolds for blood vessels.
作者
周媛
刘桂阳
ZHOU Yuan;LIU Guiyang(Textiles & Fashion, Jiangsu College of Engineering and Technology, Nantong 226006)
出处
《材料导报》
EI
CAS
CSCD
北大核心
2018年第A02期183-187,共5页
Materials Reports
基金
南通市科技计划项目(GY12016039)
江苏工院自然科学研究基金(GYKY/2016/10)
中国纺织工业联合会科技指导性项目计划(2018013)
江苏省高校"青蓝工程"资助项目
