摘要
目的:探讨髓系触发受体-1(TREM-1)的拮抗剂对大鼠肠巨噬细胞超微结构的影响。方法:选择健康雄性SD大鼠分离肠巨噬细胞,鉴定完毕后分为3组,对照组未加LPS和LP17; LPS组的LPS给药浓度为1 mg/L,治疗组LPS给药浓度为1 mg/L,LP17给药浓度为0. 1 mg/L。MTT法检测细胞活力,流式细胞仪检测细胞凋亡,Western blot检测细胞TREM-1和TNF-α蛋白表达,电镜观察细胞超微结构。结果:大鼠肠巨噬细胞生长状态良好,免疫荧光显示为绿色荧光,CD14表达阳性,细胞分布较均匀。治疗组与对照组的细胞活力指数显著高于LPS组(P <0. 05),细胞凋亡指数显著低于LPS组(P <0. 05),TREM-1、TNF-α相对表达量显著低于LPS组(P <0. 05),治疗组与对照组对比差异无统计学意义(P> 0. 05)。电镜下观察对照组肠微绒毛整齐排列,巨噬细胞形态规则; LPS组发现肠巨噬细胞内出现大量凋亡小体,巨噬细胞体积增大;治疗组发现肠巨噬细胞内出现邵爱玲凋亡小体,巨噬细胞表面伪足少。结论:TREM-1特异性拮抗剂LP17能有效抑制巨噬细胞TREM-1与TNF-α的表达,减少巨噬细胞凋亡,提高细胞活力。
Objective:To investigate the effects of antagonist of triggering receptor expressed on myeloid cells-1(TREM-1)on the ultrastructure of intestinal macrophages in rats.Methods:The isolated intestinal macrophages were separated from healthy male SD rats and were divided into three groups after identification.The control group were not added LPS and LP17,the LPS group were added LPS of 1 mg/L,the treatment group were added LPS of 1 mg/L and LP17 of 0.1mg/L.The cell viability were detected by MTT,the apoptosis were detected by flow cytometry,the expression of TREM-1 and TNF-ɑprotein were detected by Western blot,and the ultrastructure of the cells were observed by electron microscope.Results:The intestinal macrophages were grown well in rats,the immunofluorescence showed green fluorescence,and there were positive expression of CD14 and homogeneous distribution of cells.The cell viability index of the treatment group and the control group were significantly higher than that of the LPS group(P<0.05),the apoptotic index were significantly lower than that of the LPS group(P<0.05),and the relative expression of TREM-1 and TNF-ɑwere significantly lower than that of the LPS group(P<0.05),and there were no significant difference compared between the treatment group and the control group(P>0.05).Under the electron microscope,the intestinal microvilli in the control group were arranged regularly and the morphology of macrophages were regular,there were a large number of apoptotic bodies were found in the LPS group and the volume of macrophages increased,the apoptotic body in the intestinal macrophage were found in the treatment group and the surface of the macrophage were small on the surface of the macrophage.Conclusion:The TREM-1 antagonist LP17 can effectively inhibit macrophage TREM-1 and TNF-ɑexpression,reduce macrophage apoptosis and enhance cell viability.
作者
盛竹鸽
郭剑
梁爱玲
王宇晖
朱浪潮
王莹
刘利平
SHENG Zhu-ge;GUO Jian;LIANG Ai-ling;WANG Yu-hui;ZHU Lang-chao;WANG Ying;LIU Li-ping(Department of Gastroenterology,Weinan Central Hospital,Weinan 714000,Shaanxi,China)
出处
《川北医学院学报》
CAS
2018年第6期815-818,共4页
Journal of North Sichuan Medical College
基金
陕西省社会发展科技攻关项目(2016SF-013)
关键词
髓系触发受体-1
拮抗剂
肠巨噬细胞
超微结构
Triggering receptor expressed on myeloid cells-1
Antagonist
Intestinal macrophages
Ultrastructure
