摘要
以天津某大型猪场病死青年猪为研究对象,综合猪只脏器病变状况观察,以及形态学鉴定、培养基鉴定、生化鉴定和16S rRNA扩增PCR鉴定等4种方法,并通过动物致病性试验验证,结果显示,该发病猪为猪胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)感染。药敏试验结果显示,猪胸膜肺炎放线杆菌对头孢哌酮敏感;对氯霉素、阿莫西林、环丙沙星为中度敏感;对强力霉素、链霉素、卡那霉素、青霉素、四环素、氨苄青霉素、万古霉素、庆大霉素耐药。本试验为猪场猪胸膜肺炎的临床诊断及用药提供了理论依据。
In this study, three young pigs in a large pig farm from Tianjin were selected as the research objects. The results of the pathological changes of the organs, the isolation and identification of morphology, the culture medium, the biochemistry, 16 S rRNA amplification and PCR were carried out to identify pathogenic bacteria. The bacteria were identified as pathogenic bacteria by animal pathogenicity test. The results showed that the cause of swine’s death was Actinobacillus pleuropneumoniae. Then drug sensitivity test showed that APP was sensitive to cefoperazone; moderately sensitive to chloramphenicol, amoxicillin and ciprofloxacin; and resistantto doxycycline, streptomycin, kanamycin, penicillin, tetracycline, ampicillin, vancomycin, and gentamicin. This experiment provides a theoretical basis for the clinical diagnosis and medication of APP.
作者
王宇慧
李昕
孙晓莉
孟佳丽
尚翠玲
林静
金天明
WANG Yu-hui;LI Xin;SUN Xiao-li;MENG Jia-li;SHANG Cui-ling;LIN Jing;JIN Tian-ming(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2018年第4期33-37,共5页
Journal of Tianjin Agricultural University
基金
天津市农委农业科技示范推广项目(201601380)
天津市科技计划项目(高端兽药先进制造科技重大专项)(17ZXGSNC00030)
大学生创新创业训练计划项目(201710061107)
关键词
猪胸膜肺炎放线杆菌
16SrRNA
分离鉴定
药敏试验
Actinobacillus pleuropneumoniae
16S rRNA
isolation and identification
drug sensitivity test
