摘要
目的研究艾灸豚鼠血清对体外培养人黑素细胞凋亡和周期的影响,为艾灸治疗白癜风提供实验依据。方法将20只清洁级背部黄褐色毛豚鼠随机分为2组,分别为对照组、艾灸组,每组各10只。艾灸组豚鼠用自制艾灸架固定,艾灸"灸癜风""阿是穴",每日1次;对照组豚鼠同法固定,但不进行艾灸。1个月后2组豚鼠心脏采血,收集血清。体外培养人黑素细胞,分别用5%、10%、15%艾灸血清、对照血清培养24、48、72 h,用流式细胞仪检测细胞凋亡和周期。结果艾灸血清与对照血清相比,可明显降低人黑素细胞晚期凋亡率(P <0. 05)。艾灸血清对人黑素细胞早期凋亡及细胞周期无明显影响(P> 0. 05)。结论艾灸豚鼠血清可影响人黑素细胞晚期凋亡,并促进人黑素细胞增殖。
Objective To observe the effects of moxibustion guinea pig serum on cell cycle and apoptosis of human melanocytes in vitro,and to provide scientific basis for moxibustion treatment of vitiligo.Methods 20 guinea pigs with clean-grade tan fur were randomly divided into control group and moxibustion group,with 10 rats in each group.The Guinea pigs in moxibustion group were fixed with self-made moxibustion frame,and was given the moxibustion on the points of "Jiudianfeng" and "Ashi" once a day.The guinea pigs in control group were fixed with the same method,but without moxibustion treatment.One month later,two groups of guinea pigs were taken for blood collection and serum collection.Human melanocytes were cultured in vitro,cultured with 5%,10%,15% moxibustion serum and control serum for 24,48,and 72 h,respectively.Cell cycle and apoptosis were detected by flow cytometry.Results Compared with the control serum,moxibustion serum significantly reduced the late apoptotic rate of human melanocytes (P<0.05).Moxibustion serum had no significant effect on the early apoptosis and cell cycle of human melanocytes (P>0.05).Conclusion Moxibustion guinea pig serum can affect the late apoptosis of human melanocytes and promote the proliferation of human melanocytes.
作者
成玉
牛天慧
郭广进
赵广
周冬梅
张苍
曲剑华
CHENG Yu;NIU Tianhui;GUO Guangjin(Department of Dermatology,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010)
出处
《河北中医》
2018年第12期1862-1868,共7页
Hebei Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81473686)
关键词
黑素细胞
白癜风
细胞周期
针灸疗法
豚鼠
Melanocytes
Vitiligo
Cell cycle
Acupuncture therapy
Guinea pig
