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HIV抗体初筛阳性标本的复检与确证结果分析 被引量:9

Analysis of re-inspection and confirmation for HIV-antibody preliminary screening positive samples
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摘要 目的:探讨HIV抗体初筛阳性标本的复检与确证试验结果之间的关系。方法:对2011年1月至2017年10月HIV抗体初筛阳性标本按照《全国艾滋病检测技术规范(2009年、2015年修订版)》要求分别采用酶联免疫吸附试验(ELISA)和胶体硒法进行复检,复检任一方法阳性采用Western blot法进行确证,收集数据进行分析。结果:656份HIV抗体初筛阳性标本,622份复检阳性,初筛假阳性率为5.2%(34/656)。其中受检者信息齐全的490份标本进行确证试验,确证阳性标本450份(91.8%),阴性11份(2.3%),不确定29份(5.9%)。ELISA法复检阳性标本确证阳性率为92.9%(450/488),胶体硒法复检阳性标本确证阳性率为99.1%(450/454),差异有统计学意义(χ~2=31.22,P<0.01);ELISA法中S/CO值>6组与3≤S/CO值≤6组和1≤S/CO值<3组的确证阳性率比较差异均有统计学意义(χ~2=96.33、301.15,P<0.01);ELISA法S/CO值与确证阳性标本出现条带数量呈正相关,Gamma系数为0.788。结论:采用优先选择胶体硒法+ELISA法补充的模式进行复检可以大大提高检测效率,保证及时有效地发现HIV感染者。较高S/CO值的标本感染HIV的可能性较大,且病毒处于复制活跃期具有较强感染性的可能性也较大。 Objective:To analyze the correlation between the re-inspection and confirmation for HIV-antibody preliminary screening positive samples.Method:ELISA and colloidal selenium assay were used to re-inspect preliminarily screening positive samples from Jan 2011 to Oct 2017,then the suspicious re-inspection positive samples were confirmed by Western blot.Results: There were 656 HIV-antibody preliminary screening positive samples in total,and 622 re-inspection samples were positive.The false positive rate of HIV-antibody preliminary screening was 5.2%(34/656).And 490 samples with complete information of the subjects were confirmed by Western blot,and 450 samples were positive(91.8%),11 samples were negative(2.3%),and 29 samples were uncertain(5.9%).The confirmed positive rate was 92.9%(450/488)in samples re-inspected by ELISA,and the confirmed positive rate was 99.1%(450/454)in samples re-inspected by colloidal selenium assay(P<0.01).Compared with the S/ CO>6 group,the confirmed positive rates of the 3≤S/CO≤6 grouPand 1≤S/CO<3 grouPin samples re-inspected by ELISA were significantly lower(P<0.01).The S/CO value was positively correlated with the number of bands of confirmed positive samples(P<0.01).Conclusion:Application of colloidal selenium assay combined with ELISA can greatly increase the confirmed positive rate.
作者 周昀 杨新智 周秋颖 王建华 孙玉萍 杨政权 ZHOU Yun;YANG Xinzhi;ZHOU Qiuying;WANG Jianhua;SUN Yuping;YANG Zhengquan(Dandong Disease Prevention and Control Center,Dandong 118000,China)
出处 《沈阳医学院学报》 2019年第1期56-58,61,共4页 Journal of Shenyang Medical College
关键词 HIV抗体 筛查阳性 确证试验 Western BLOT法 HIV antibody screening positive confirmatory testing Western blot
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