摘要
目的:探讨风湿性心脏病合并心房颤动患者右心耳组织中LncRNA表达变化,为进一步研究心房颤动发生机理提供实验依据。方法:选择风湿性心脏病合并心房颤动患者5例和风湿性心脏病未合并有心房颤动患者5例,外科手术中切下的右心耳组织作为实验样本,分别作为实验组及对照组,采用高通量基因芯片技术检测,获得LncRNA和mRNA差异表达数据,对差异表达的m RNAs进行GO、KEGG等初步生物信息学分析。结果:与对照组LncRNA基因表达谱相比,实验组LncRNA表达水平显著上调有119个(其中有9个Fold change>4倍),表达水平下调有214个(其中有14个Fold change>4倍);Go分析结果中,生物学过程差异基因富集条目169条,细胞组分差异基因富集条目18条,分子生物学差异基因富集条目89条;KEGG分析显示,受差异基因调控通路共18条。结论:LncRNA在房颤患者右心耳组织中存在差异表达,提示LncRNA可能参与房颤发生与发展的调控,并将为进一步研究提供实验依据。
Objective:To investigate the change in the expression of long non-coding RNA(lncRNA)in the auricula dextra in patients with rheumatic heart disease and atrial fibrillation(AF),and to provide an experimental basis for further research on the pathogenesis of AF.Methods:A total of 5 patients with rheumatic heart disease and AF and 5 with rheumatic heart disease alone were enrolled.The auricula dextra tissue samples were collected during surgery and were divided into experimental group and control group.High-throughput gene microarray was used to obtain the data on the differential expression of lncRNA and mRNA,and gene ontology(GO)and Kyoto Encyclopedia of Genes and Genome(KEGG)were used to perform a bioinformatics analysis of differentially expressed mRNAs.Results:Compared with the control group,the experimental group had 119 significantly upregulated lncRNAs(among which 9 had a fold change of>4 times)and 214 significantly downregulated lncRNAs(among which 14 had a fold change of>4 times).GO analysis showed 169 significantly enriched biological process terms,18 significantly enriched cellular component terms,and 89 significantly enriched molecular biology terms.KEGG analyses showed that 18 signaling pathways were regulated by differentially expressed genes.Conclusion:There is differential expression of lncRNAs in the auricula dextra tissue in patients with AF,suggesting that lncRNA might be involved in the regulation of the development and progression of AF,which will provide an experimental basis for further studies.
作者
董亚辉
杨探
李秋芳
李光
万居易
聂永梅
于风旭
DONG Yahui;YANG Tan;LI Qiufang;LI Guang;WAN Juyi;NIE Yongmei;YU Fengxu(Department of Cardiovascular Surgery,the Affiliated Hospital of Southwest Medical University,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Key Laboratory of Medical Electrophysiology,Ministry of Education of China,Southwest Medical University,Luzhou 646000,Sichuan Province,China)
出处
《西南医科大学学报》
2019年第1期52-57,共6页
Journal of Southwest Medical University
基金
泸州-西南医科大联合基金(2015SX-0029)
四川省科技厅重点项目(2018JY0080)
关键词
心房颤动
LncRNA
基因表达
GENE
Ontology分析
KEGG分析
Atrial fibrillation
Long non-coding RNA
Gene expression
Gene Ontology analysis
Kyoto Encyclopedia of Genes and Genome analysis
