摘要
目的探讨7,8-二羟基黄酮(7,8-DHF)与施万细胞(SCs)对大鼠脱细胞同种异体神经支架(ANA)移植坐骨神经缺损后轴突再生和功能恢复的影响。方法 45只成年SD大鼠随机分为脱细胞神经支架(ANA)组、SCs组和7,8-DHF+SCs组。采用坐骨神经功能指数(SFI)和电生理方法检测运动功能的恢复,Western bolt检测再生神经内BDNF及其受体TrkB的蛋白表达,免疫荧光示踪PKH26标记的移植SCs,NF和S100免疫荧光法检测再生神经内轴突再生和髓鞘形成情况。结果与SCs组比较,7,8-DHF+SCs组再生神经内BDNF和TrkB蛋白相对表达明显增高,PKH-26标记的SCs数量显著增高,NF和S100表达增强(P<0. 05)。与对照组比较,SCs组和7,8-DHF+SCs组神经传导速度增快、波幅增高、延迟期缩短、SFI指数增高,其中7,8-DHF+SCs组电生理和SFI指标优于SCs组(P<0. 05)。结论 7,8-DHF联合SCs对坐骨神经损伤后轴突再生、髓鞘形成和功能恢复的作用优于SCs组,其机制可能与7,8-DHF促进再生神经BDNF及其受体TrkB表达,进而促进移植的SCs存活有关。
Objective To study the effect of 7,8-Dihydroxyflavone(7,8-DHF)and Schwann cells(SCs)transplantation on axon regeneration and function recovery after acellular nerve allograft(ANA)repair of the sciatic nerve gap in mice.Methods A total of 45 healthy SD rat were randomly divided into control group,SCs group and 7,8-DHF+SCs group.SFI and electrophysiology were used to evaluate functional recovery.BDNF and TrkB protein were evaluated by western bolt,observe the fluorescence signal of SCs labeled with PKH-26 by using immunofluorescence,observe the axonal regeneration and myelination by using NF,S100 immunofluorescence.Results Compared with SCs group,the expression of BDNF,TrkB,NF,S100 and PKH-26 labeled SCs in 7,8-DHF+SCs group were increased(P<0.05).Compared with control group,nerve conduction velocity and wave amplitude were significantly increased in SCs group and 7,8-DHF+SCs group(P<0.05),however,incubation period were significantly decreased in two treatment group(P<0.05),the effect of 7,8-DHF+SCs group was more powerful than SCs group(P<0.05),moreover,the SFI score of 7,8-DHF+SCs group was higher than control group and SCs group.Conclusion The combined 7,8-DHF and SCs transplantation treatment promoted axonal regeneration,myelination and function recovery significantly more than SCs treatments,which may be related to 7,8-DHF upregulating BDNF and TrkB expression in the ANA,and promoting SCs survival.
作者
秦涛
王志龙
廉洪宇
马遇伯
QIN Tao;WANG Zhilong;LIAN Hongyu;MA Yubo(Second Department of Orthopaedics,Hongqi Hospital affiliated to Mudanjiang Medical College,Mudanjiang 157011,Heilongjiang Province;Graduate cultivation Department of Hongqi Hospital affiliated to Mudanjiang Medical College Heilongjiang Mudanjiang 157011)
出处
《中国实用神经疾病杂志》
2018年第22期2437-2443,共7页
Chinese Journal of Practical Nervous Diseases
