摘要
目的:建立一种量化的染色体制片质量评价标准。方法:随机选取2016年9月—2017年3月于深圳市龙岗区妇幼保健院行外周血染色体检测的受检者233例,同时使用常规方法以及高分辨制片法进行细胞培养、制片及核型分析。通过核型中15个质评条带能否清晰识别对每个样本进行制片效果的评分,比较中低分辨组和高分辨组的评分。结果:高分辨方法评分高于中低分辨方法,差异有统计学意义(P<0.05)。高分辨组染色体形态清晰,带纹丰富,长度适中,分散适度。结论:基于15条质评条带进行质量评价可量化地评估染色体的制片效果,便于染色体核型分析的室内质控和室间质评,具有临床应用价值。
Objective:To develop a quantitative evaluation method for G-band chromosome preparation.Methods:233 subjects were randomly selected for peripheral blood karyotyping analysis in our hospital from Sep 2016 to Mar 2017.For each sample,both conventional method and high resolution chromosome method were used for cell culture,harvest and karyotype analysis.Quality evaluation score was obtained using 15 quality assessment bands.This score was then compared between the low-median resolution group and the high resolution group.Results:The score of high resolution group was higher than that of group (P<0.05).The chromosomes of high resolution group were clear,rich in stripes,moderate in length and dispersion.Conclusions:The quality evaluation based on 15 quality assessment bands can be used to quantify the effect of chromosome preparation,as the internal quality control in laboratory and external quality assessment among laboratories of chromosome karyotyping analysis,suggesting its clinical value in the diagnosis of chromosomal diseases.
作者
胡亮
罗小金
丛潇怡
裴元元
魏凤香
HU Liang;LUO Xiao-jin;CONG Xiao-yi;PEI Yuan-yuan;WEI Feng-xiang(Shenzhen Longgang Maternal and Child Health Hospital,Shenzhen 518172,Guangdong Province,China)
出处
《国际生殖健康／计划生育杂志》
CAS
2019年第1期53-55,共3页
Journal of International Reproductive Health/Family Planning
基金
深圳市龙岗区医疗卫生科技计划项目(20170403105938224)
